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<p>Diseases are a global threat to rice production. Among them, rice blast and bacterial blight (BB) are most significant. To enhance targeted disease resistance, the marker-assisted backcross breeding (MABB) approach was employed to pyramid two blast (<i>Pi9</i> and <i>Pb1</i>) and three BB resistant (R) genes (<i>Xa4</i>, <i>xa13</i>, and <i>Xa21</i>) into a high-quality rice cultivar BRRI dhan100. The donor parents Pi9-US2 (<i>Pi9</i>), Pb1-US2 (<i>Pb1</i>), and IRBB58 (<i>Xa4</i>, <i>xa13</i>, and <i>Xa21</i>) contributed these five genes. We followed a triple-cross breeding strategy, followed by backcrossing, selfing, and foreground selection to produce BC<sub>3</sub>F<sub>5</sub> progenies. Chi-square analysis of 420 BC<sub>3</sub>F<sub>2</sub> individuals confirmed the inheritance patterns of blast and BB resistance were controlled by a simple Mendelian fashion. Finally, we selected 38 advanced lines (ALs), and among them, twelve lines possessed all 5 candidate R genes, while fifteen consisted of 4 genes in different combinations. The disease rating scale of these ALs varied from 0 to 3 for both blast and BB diseases, while BRRI dhan100 ranging from 7 to 9. The G23, G13, G5, G10, G15, and G29 ALs exhibited higher yields ranging from 7.22–7.34 ton ha<sup>−1</sup> compared to the check, BRRI dhan100. Marker-trait association analysis displayed molecular markers negatively associated with disease susceptibility. Therefore, gene introgression by MABB could effectively identify and functionally validate the candidate R genes with high accuracy to develop a resistant variety to multiple diseases in rice breeding programs.</p>

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Gene pyramiding for enhancing resistance to blast and bacterial blight disease in rice through maker assisted selection

  • 期刊论文

  • 发表日期：

    2025年9月16日

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  • Mohammad Abdul Latif,
  • Omar Kayess,
  • Rakibul Hasan

摘要

Diseases are a global threat to rice production. Among them, rice blast and bacterial blight (BB) are most significant. To enhance targeted disease resistance, the marker-assisted backcross breeding (MABB) approach was employed to pyramid two blast (Pi9 and Pb1) and three BB resistant (R) genes (Xa4, xa13, and Xa21) into a high-quality rice cultivar BRRI dhan100. The donor parents Pi9-US2 (Pi9), Pb1-US2 (Pb1), and IRBB58 (Xa4, xa13, and Xa21) contributed these five genes. We followed a triple-cross breeding strategy, followed by backcrossing, selfing, and foreground selection to produce BC3F5 progenies. Chi-square analysis of 420 BC3F2 individuals confirmed the inheritance patterns of blast and BB resistance were controlled by a simple Mendelian fashion. Finally, we selected 38 advanced lines (ALs), and among them, twelve lines possessed all 5 candidate R genes, while fifteen consisted of 4 genes in different combinations. The disease rating scale of these ALs varied from 0 to 3 for both blast and BB diseases, while BRRI dhan100 ranging from 7 to 9. The G23, G13, G5, G10, G15, and G29 ALs exhibited higher yields ranging from 7.22–7.34 ton ha−1 compared to the check, BRRI dhan100. Marker-trait association analysis displayed molecular markers negatively associated with disease susceptibility. Therefore, gene introgression by MABB could effectively identify and functionally validate the candidate R genes with high accuracy to develop a resistant variety to multiple diseases in rice breeding programs.