Scholarlink

Objectives <p>Expressing cellulase systems in <i>C. glycerinogenes</i> with extracellular secretion ability, and using fermentation with residue, enabled the recombinant strain to degrade lignocellulosic waste for efficient glycerol production, offering new option for agricultural waste transformation.</p> Results <p><i>Candida glycerinogenes</i> is employed as the host strain, various cellulases were screened. Signal peptide mining and screening, and semi-rational design strategy was adopted in the host strain. The recombinant strain Cg4 had a total cellulase activity of 13.6 U/mL. Cg4 was applied to 110 g/L sugarcane bagasse hydrolysate with 40 g/L of pretreated bagasse as the substrate. The glycerol yield reached 50 g/L, with a 43.3% bagasse utilization rate, the amount of cellulase used in the degradation of lignocellulose was reduced by 12.1%.</p> Conclusion <p>Opened up a route for efficient degradation of agricultural waste of lignocellulose by <i>C. glycerinogenes</i> for glycerol production, the emission of lignocellulose waste had been reduced, and decreasing the need for cellulases in hydrolysis.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Expression of cellulase in Candida glycerogenes and strengthen the expression level for application in residue-containing fermentation to enhance glycerol production

  • 期刊论文

  • 发表日期：

    2025年6月27日

查看全文
  • Yuxin Gao,
  • Dongqi Jiang,
  • Mengying Wang,
  • Xueqing Du,
  • Hong Zong,
  • Bin Zhuge

摘要

Objectives

Expressing cellulase systems in C. glycerinogenes with extracellular secretion ability, and using fermentation with residue, enabled the recombinant strain to degrade lignocellulosic waste for efficient glycerol production, offering new option for agricultural waste transformation.

Results

Candida glycerinogenes is employed as the host strain, various cellulases were screened. Signal peptide mining and screening, and semi-rational design strategy was adopted in the host strain. The recombinant strain Cg4 had a total cellulase activity of 13.6 U/mL. Cg4 was applied to 110 g/L sugarcane bagasse hydrolysate with 40 g/L of pretreated bagasse as the substrate. The glycerol yield reached 50 g/L, with a 43.3% bagasse utilization rate, the amount of cellulase used in the degradation of lignocellulose was reduced by 12.1%.

Conclusion

Opened up a route for efficient degradation of agricultural waste of lignocellulose by C. glycerinogenes for glycerol production, the emission of lignocellulose waste had been reduced, and decreasing the need for cellulases in hydrolysis.