Scholarlink

<p>Squalene is a triterpene with various biological applications. However, the conventioneer squalene industry is limited by complex extraction processes and environmental pollution, necessitating an environmentally sustainable solution to the increasing demand for squalene. Microbial synthesis is a potentially green and efficient method of producing squalene. Acetyl-CoA is a key precursor of squalene. First, we investigated the effects of enhanced acetyl-CoA supply on squalene production, lipid content, and total fatty acid content in <i>Yarrowia lipolytica</i>. Then, strain YLACLH2 with a squalene production of 232.29&#xa0;mg/L was obtained by co-overexpressing YlACL2 and YlHMG1. Subsequently, the squalene production of YLACLH2 was increased to 514.33&#xa0;mg/L by fermentation engineering, optimizing fermentation conditions including temperature, media volume, C/N ratio, shaker flask type and medium type. Finally, we investigated the synthesis efficiency of squalene in <i>Y. lipolytica</i> by acid-hydrolyzed sugarcane molasses (AHM) and waste cooking oil (WCO) as carbon sources with optimized fermentation conditions. This study showed that <i>Y. lipolytica</i> has the potential to produce squalene industrially using low-cost substrates. Our study findings provide reference for engineering <i>Y. lipolytica</i> to produce squalene using low-cost substrates and in an environmentally sustainable manner.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Cost-effective production of squalene using Yarrowia lipolytica via metabolic engineering and fermentation engineering

  • 期刊论文

  • 发表日期：

    2025年5月5日

查看全文
  • Wen-Bo Lin,
  • Hong Chen,
  • Ze-Qi Song,
  • Yu-Qing Pan,
  • Peng-Cheng Hu,
  • Xiao-Na Yang,
  • Xiang-Yang Lu,
  • Yun Tian,
  • Hu-Hu Liu

摘要

Squalene is a triterpene with various biological applications. However, the conventioneer squalene industry is limited by complex extraction processes and environmental pollution, necessitating an environmentally sustainable solution to the increasing demand for squalene. Microbial synthesis is a potentially green and efficient method of producing squalene. Acetyl-CoA is a key precursor of squalene. First, we investigated the effects of enhanced acetyl-CoA supply on squalene production, lipid content, and total fatty acid content in Yarrowia lipolytica. Then, strain YLACLH2 with a squalene production of 232.29 mg/L was obtained by co-overexpressing YlACL2 and YlHMG1. Subsequently, the squalene production of YLACLH2 was increased to 514.33 mg/L by fermentation engineering, optimizing fermentation conditions including temperature, media volume, C/N ratio, shaker flask type and medium type. Finally, we investigated the synthesis efficiency of squalene in Y. lipolytica by acid-hydrolyzed sugarcane molasses (AHM) and waste cooking oil (WCO) as carbon sources with optimized fermentation conditions. This study showed that Y. lipolytica has the potential to produce squalene industrially using low-cost substrates. Our study findings provide reference for engineering Y. lipolytica to produce squalene using low-cost substrates and in an environmentally sustainable manner.