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<p><i>Tussilago farfara</i> L<i>.</i>, a member of the Asteraceae family, is an economically valuable species due to its edible and medicinal properties. To elucidate the structural characteristics, genetic mechanisms, and evolutionary pathways of the organelle genomes of <i>T. farfara</i>, we sequenced, assembled, and annotated its mitochondrial genome for the first time. The complete mitochondrial genome of <i>T. farfara</i> spans 306,024&#xa0;bp and contains 33 mitochondrial protein-coding genes (PCGs), 3 rRNAs, and 22 tRNAs. Analysis of the nucleotide substitution rate and genetic diversity revealed that most mitochondrial genome genes may have undergone purifying selection, indicating a slow evolutionary rate and a relatively conserved genomic structure. We further identified 13 fragments of chloroplast-derived DNA integrated into the mitochondrial genome, evidencing intracellular gene transfer. Collinearity analysis showed that <i>Arctium lappa</i> shares the most extensive mitochondrial homologous sequences and the highest sequence similarity with <i>T. farfara</i>. Phylogenetic analysis based on the mitochondrial genome helped to clarify the evolutionary and taxonomic position of <i>T. farfara</i> within the Asteraceae family. The mitochondrial genome sequence of <i>T. farfara</i> provides a valuable genomic resource for species identification and for evolutionary studies within the Asteraceae family.</p> Graphical Abstract <p></p>

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Assembly and Characterization of the First Complete Mitochondrial Genome of Tussilago farfara L.: Insights into Biological Functions and Phylogenetic Relationships within the Asteraceae Family

  • 期刊论文

  • 发表日期：

    2026年2月26日

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  • Yilei Zhang,
  • Yonggang Yan,
  • Gang Zhang,
  • Ying Chen,
  • Yimin Li,
  • Jing Gao,
  • Bingyue Yang,
  • Liang Peng

摘要

Tussilago farfara L., a member of the Asteraceae family, is an economically valuable species due to its edible and medicinal properties. To elucidate the structural characteristics, genetic mechanisms, and evolutionary pathways of the organelle genomes of T. farfara, we sequenced, assembled, and annotated its mitochondrial genome for the first time. The complete mitochondrial genome of T. farfara spans 306,024 bp and contains 33 mitochondrial protein-coding genes (PCGs), 3 rRNAs, and 22 tRNAs. Analysis of the nucleotide substitution rate and genetic diversity revealed that most mitochondrial genome genes may have undergone purifying selection, indicating a slow evolutionary rate and a relatively conserved genomic structure. We further identified 13 fragments of chloroplast-derived DNA integrated into the mitochondrial genome, evidencing intracellular gene transfer. Collinearity analysis showed that Arctium lappa shares the most extensive mitochondrial homologous sequences and the highest sequence similarity with T. farfara. Phylogenetic analysis based on the mitochondrial genome helped to clarify the evolutionary and taxonomic position of T. farfara within the Asteraceae family. The mitochondrial genome sequence of T. farfara provides a valuable genomic resource for species identification and for evolutionary studies within the Asteraceae family.

Graphical Abstract