Scholarlink

<p><i>Streptomyces</i> spp. is a crucial source for the drug discovery and development, especially those from extreme environments. The complete genome was sequenced to uncover the biosynthetic potential of the strain Qhu-M197 producing mithramycin isolated from the alpine meadow of the Qinghai-Tibetan Plateau. The results revealed that the genome consists of one linear chromosome and two plasmids with the size of 9.12 megabases (Mb) and the guanine-cytosine (G + C) content of 71.52%. The phylogenetic analyses of 16S rRNA gene and genome of strain Qhu-M197 suggested the strain belonged to <i>Streptomyces</i> genus, and the closest phylogenetic relationship between the strain Qhu-M197 and <i>S. phaeoluteigriseus</i> DSM 41896<sup>T</sup>. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between Qhu-M197 and <i>S. phaeoluteigriseus</i> DSM 41896<sup>T</sup> are 97.47% and 84.1%, respectively, indicating that they likely belong to the same species. However, comparative genomic analysis with closely related species reveals that Qhu-M197 may represent a novel strain of <i>S. phaeoluteigriseus</i>, highlighting its potential distinction at the strain level. The biosynthetic gene clusters (BGCs) analyzed by antiSMASH v7.0 revealed that Qhu-M197 had 33 BGCs encoding secondary metabolites. Interestingly, about 64% BGCs show less than 50% similarity to those annotated, which provides new opportunities for the discovery of novel natural products.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Complete Genome Sequencing of Streptomyces sp. Qhu-M197 Isolated from Alpine Meadows in the Qinghai-Tibetan Plateau Uncovers Biosynthetic Gene Clusters Encoding Bioactive Natural Products

  • 期刊论文

  • 发表日期：

    2026年1月8日

查看全文
  • Xin Xiang,
  • Jiakai Fan,
  • Xingyu Liu,
  • Guangzhi Li,
  • Chenghang Sun,
  • Benyin Zhang

摘要

Streptomyces spp. is a crucial source for the drug discovery and development, especially those from extreme environments. The complete genome was sequenced to uncover the biosynthetic potential of the strain Qhu-M197 producing mithramycin isolated from the alpine meadow of the Qinghai-Tibetan Plateau. The results revealed that the genome consists of one linear chromosome and two plasmids with the size of 9.12 megabases (Mb) and the guanine-cytosine (G + C) content of 71.52%. The phylogenetic analyses of 16S rRNA gene and genome of strain Qhu-M197 suggested the strain belonged to Streptomyces genus, and the closest phylogenetic relationship between the strain Qhu-M197 and S. phaeoluteigriseus DSM 41896T. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between Qhu-M197 and S. phaeoluteigriseus DSM 41896T are 97.47% and 84.1%, respectively, indicating that they likely belong to the same species. However, comparative genomic analysis with closely related species reveals that Qhu-M197 may represent a novel strain of S. phaeoluteigriseus, highlighting its potential distinction at the strain level. The biosynthetic gene clusters (BGCs) analyzed by antiSMASH v7.0 revealed that Qhu-M197 had 33 BGCs encoding secondary metabolites. Interestingly, about 64% BGCs show less than 50% similarity to those annotated, which provides new opportunities for the discovery of novel natural products.