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<p>Esophageal carcinoma (ESCA) represents a highly aggressive malignancy with limited therapeutic options. Mounting evidence indicated that Nuclear Lamin B2 (LMNB2) was involved in tumor progression through modulation of multiple signaling pathways. However, the investigation of LMNB2 role in tumor cell stemness and the Warburg effect remains inconclusive. This study utilized the UALCAN database to perform pan-cancer, clinical sample and cell line analyses. Western blot validation demonstrated substantial LMNB2 overexpression in ESCA cells. Silencing LMNB2 expression significantly repressed ESCA cells stemness and Warburg effect. Integrated bioinformatics analyses using Linkedomics, Funrich, and String databases identified the p38 MAPK pathway as a downstream mediator. In vitro experiments verified the regulatory impact of LMNB2 on the p38 phosphorylation in the MAPK signaling pathway, implicated in the regulation of tumor sphere formation and the Warburg effect. LMNB2 also exhibited a regulatory role in tumor growth within the mouse xenograft model. This study proposed that LMNB2 could emerge as a novel therapeutic strategy for treating ESCA.</p>

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LMNB2 Regulates Esophageal Carcinoma Stemness and Warburg Effect by Modulating the p38 MAPK Signaling Pathway

  • 期刊论文

  • 发表日期：

    2025年11月14日

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  • Xiaofeng Zhu,
  • Xiaowen Zhao,
  • Yayun Cui

摘要

Esophageal carcinoma (ESCA) represents a highly aggressive malignancy with limited therapeutic options. Mounting evidence indicated that Nuclear Lamin B2 (LMNB2) was involved in tumor progression through modulation of multiple signaling pathways. However, the investigation of LMNB2 role in tumor cell stemness and the Warburg effect remains inconclusive. This study utilized the UALCAN database to perform pan-cancer, clinical sample and cell line analyses. Western blot validation demonstrated substantial LMNB2 overexpression in ESCA cells. Silencing LMNB2 expression significantly repressed ESCA cells stemness and Warburg effect. Integrated bioinformatics analyses using Linkedomics, Funrich, and String databases identified the p38 MAPK pathway as a downstream mediator. In vitro experiments verified the regulatory impact of LMNB2 on the p38 phosphorylation in the MAPK signaling pathway, implicated in the regulation of tumor sphere formation and the Warburg effect. LMNB2 also exhibited a regulatory role in tumor growth within the mouse xenograft model. This study proposed that LMNB2 could emerge as a novel therapeutic strategy for treating ESCA.