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<p>MiR-195-5p has been confirmed to be intimately linked to many cancers development, whereas its role in gastric cancer (GC) remains unelucidated. The objective of this study was to explore the potential function and mechanism of miR-195-5p in GC. First, miR-195-5p level in GC was analyzed based on clinical specimens and TCGA database. Then, the function of miR-195-5p in GC cells was detected via CCK-8, clone formation assay, flow cytometry, transwell, and wound healing assay. Subsequently, we explored the possible mechanism of miR-195-5p using KEGG analysis, database analysis, Western blot, and rescue assays. In this research, miR-195-5p was declined in GC and associated to malignant progression of GC. The introduction of miR-195-5p weakened cell growth, metastasis, and facilitated cell apoptosis. KEGG enrichment analysis indicated that miR-195-5p influenced the malignant progression of GC through mechanisms related to autophagy. MiR-195-5p overexpression leads to a diminution in cellular autophagy. Further studies identified that miR-195-5p bound to Lysosome-associated membrane protein 2 (LAMP2). Mechanism studies confirmed that miR-195-5p introduction attenuated cell growth, and this process was rescued by LAMP2 overexpression. Collectively, our work disclosed that miR-195-5p affected the malignant progression of GC by regulating LAMP2-mediated autophagy.</p>

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Overexpression of miR-195-5p Suppresses Gastric Cancer Progression by Regulating LAMP2-Mediated Autophagy

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年5月5日

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  • Chenglong Shen,
  • Fang Zhou,
  • Xiaolei Zhang,
  • Jiazhe Shao,
  • Yuchao Wang,
  • Haichen Liu,
  • Yichen Li,
  • Guoqiang Zhou,
  • Zhiliang Shi

摘要

MiR-195-5p has been confirmed to be intimately linked to many cancers development, whereas its role in gastric cancer (GC) remains unelucidated. The objective of this study was to explore the potential function and mechanism of miR-195-5p in GC. First, miR-195-5p level in GC was analyzed based on clinical specimens and TCGA database. Then, the function of miR-195-5p in GC cells was detected via CCK-8, clone formation assay, flow cytometry, transwell, and wound healing assay. Subsequently, we explored the possible mechanism of miR-195-5p using KEGG analysis, database analysis, Western blot, and rescue assays. In this research, miR-195-5p was declined in GC and associated to malignant progression of GC. The introduction of miR-195-5p weakened cell growth, metastasis, and facilitated cell apoptosis. KEGG enrichment analysis indicated that miR-195-5p influenced the malignant progression of GC through mechanisms related to autophagy. MiR-195-5p overexpression leads to a diminution in cellular autophagy. Further studies identified that miR-195-5p bound to Lysosome-associated membrane protein 2 (LAMP2). Mechanism studies confirmed that miR-195-5p introduction attenuated cell growth, and this process was rescued by LAMP2 overexpression. Collectively, our work disclosed that miR-195-5p affected the malignant progression of GC by regulating LAMP2-mediated autophagy.