Experimental studies of semiautomatic cryoconservation process for biological tissue specimens
摘要
Local low-temperature effects on samples of sheep aorta were studied using an experimental apparatus serving as a prototype for a medical device for semi-automatic cryopreservation of biological tissue with the option of using a variety of cryoprotectants with regulation of coolant flow and temperature. The effectiveness of cell preservation was assessed by histological examination. The results showed that use of polydimethylsiloxane as a cryoprotectant led to preservation of biological tissue and has prospects for further research in order to find optimal modes of semi-automatic cryopreservation.