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<p>The efficacy of biocontrol agents against plant diseases is strongly influenced by abiotic factors, particularly temperature at the time of their application. Lack of knowledge on this effect can lead to inappropriate use of biocontrol agents, resulting in poor performance in the field. This study investigates how temperature affects the performance of a <i>Bacillus velezensis</i> strain used to control <i>Sclerotinia sclerotiorum</i> on <i>Brassica napus</i>. To this end, the effect of temperature (5, 13, 21 and 28 °C) was assessed on bacterial growth, lipopeptides production, antifungal activity of cell-free supernatants, and the protective efficacy of the bacterium on the plant. Interestingly, although the best bacterial growth was obtained at 28 °C, the highest protective efficacy against the most aggressive strain of <i>S. sclerotiorum</i> was observed at 21 °C. Following a 24&#xa0;h exposure of leaves to these temperatures, the protective efficacy was 26% (SE = 8) at 28&#xa0;°C and 61% (SE = 4) at 21&#xa0;°C. This temperature corresponds to the peak of lipopeptides production by the <i>B. velezensis</i> strain. Despite significant variation in cell-free supernatants lipopeptides levels across culture temperatures, similar inhibition was observed. This result suggests that additional metabolites may contribute to the direct inhibition of the pathogen. These results underline the importance of environmental conditions in determining the activity of biocontrol agents. Understanding these efficacy factors is crucial to improve the reliability and field performance of biocontrol strategies in agriculture.</p>

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The critical role of temperature in ensuring reliable protective efficacy of a biocontrol agent targeting Sclerotinia sclerotiorum

  • 期刊论文

  • 发表日期：

    2026年5月13日

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  • M. Grimonpont,
  • E. Turc,
  • A. Lies,
  • N. Monnier,
  • P. C. Nicot,
  • M. Bardin

摘要

The efficacy of biocontrol agents against plant diseases is strongly influenced by abiotic factors, particularly temperature at the time of their application. Lack of knowledge on this effect can lead to inappropriate use of biocontrol agents, resulting in poor performance in the field. This study investigates how temperature affects the performance of a Bacillus velezensis strain used to control Sclerotinia sclerotiorum on Brassica napus. To this end, the effect of temperature (5, 13, 21 and 28 °C) was assessed on bacterial growth, lipopeptides production, antifungal activity of cell-free supernatants, and the protective efficacy of the bacterium on the plant. Interestingly, although the best bacterial growth was obtained at 28 °C, the highest protective efficacy against the most aggressive strain of S. sclerotiorum was observed at 21 °C. Following a 24 h exposure of leaves to these temperatures, the protective efficacy was 26% (SE = 8) at 28 °C and 61% (SE = 4) at 21 °C. This temperature corresponds to the peak of lipopeptides production by the B. velezensis strain. Despite significant variation in cell-free supernatants lipopeptides levels across culture temperatures, similar inhibition was observed. This result suggests that additional metabolites may contribute to the direct inhibition of the pathogen. These results underline the importance of environmental conditions in determining the activity of biocontrol agents. Understanding these efficacy factors is crucial to improve the reliability and field performance of biocontrol strategies in agriculture.