Scholarlink

<p>Bakanae, a disease of rice caused by <i>Fusarium</i> sp., affects different growth stages, leading to heavy losses in crop production. The aim of this research was to determine the biocontrol potential of three bacterial species against two bakanae pathogens. The antagonistic activity of three bacteria (<i>Aneurinibacillus migulanus</i> Nagano, <i>Bacillus subtilis</i> MBI 600 and <i>Pseudomonas fluorescens</i> B5) were evaluated <i>in vitro</i> against two strains of <i>F. verticillioides</i> CBS299.96 and 19-115 and two of <i>F. fujikuroi</i> M1149 and VAE5 on potato dextrose agar at 25&#xa0;°C using dual culture techniques. Greatest inhibition of colony growth of all tested pathogen strains occurred in dual cultures with <i>Bacillus subtilis</i> MBI 600 (53.7–64.5%), followed by <i>A. migulanus</i> Nagano (17.6–32.7%) and <i>P. fluorescens</i> B5 (2.4–43.3%), when compared to control cultures. Subsequently, these biological control agents were tested <i>in planta</i> against the highly virulent <i>F. fujikuroi</i> VAE5. Biocontrol potential of <i>A. migulanus</i> Nagano in suppressing bakanae disease was the strongest, leading to increases in plant height, nitrogen balance index, chlorophyll balance index, root length, shoot and root biomass, by 59.6, 44.5, 55.2, 69.2, 101.6, and 90.1%, respectively, and decreased disease severity by 26.7%, when co-inoculated with the <i>F. fujikuroi</i> VAE5, compared to plants treated with the <i>F. fujikuroi</i> VAE5 alone. Application of <i>B. subtilis</i> MBI 600 and <i>P. fluorescens</i> B5 both decreased disease severity by 6.7%. These results encourage the further development of these bacteria for reducing the impact of <i>F. fujikuroi</i> VAE5 on rice plants.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Biological control of Fusarium fujikuroi, cause of bakanae disease of rice (Oryza sativa L.), using antagonistic bacteria

  • 期刊论文

  • 发表日期：

    2025年12月15日

查看全文
  • Istiaq Ahmed,
  • Gareth J. Norton,
  • Stephen Woodward

摘要

Bakanae, a disease of rice caused by Fusarium sp., affects different growth stages, leading to heavy losses in crop production. The aim of this research was to determine the biocontrol potential of three bacterial species against two bakanae pathogens. The antagonistic activity of three bacteria (Aneurinibacillus migulanus Nagano, Bacillus subtilis MBI 600 and Pseudomonas fluorescens B5) were evaluated in vitro against two strains of F. verticillioides CBS299.96 and 19-115 and two of F. fujikuroi M1149 and VAE5 on potato dextrose agar at 25 °C using dual culture techniques. Greatest inhibition of colony growth of all tested pathogen strains occurred in dual cultures with Bacillus subtilis MBI 600 (53.7–64.5%), followed by A. migulanus Nagano (17.6–32.7%) and P. fluorescens B5 (2.4–43.3%), when compared to control cultures. Subsequently, these biological control agents were tested in planta against the highly virulent F. fujikuroi VAE5. Biocontrol potential of A. migulanus Nagano in suppressing bakanae disease was the strongest, leading to increases in plant height, nitrogen balance index, chlorophyll balance index, root length, shoot and root biomass, by 59.6, 44.5, 55.2, 69.2, 101.6, and 90.1%, respectively, and decreased disease severity by 26.7%, when co-inoculated with the F. fujikuroi VAE5, compared to plants treated with the F. fujikuroi VAE5 alone. Application of B. subtilis MBI 600 and P. fluorescens B5 both decreased disease severity by 6.7%. These results encourage the further development of these bacteria for reducing the impact of F. fujikuroi VAE5 on rice plants.