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<p>Plant endophytic bacteria play crucial roles in the prevention and control of crop diseases. Yam (<i>Dioscorea opposita</i>), an important tuber and root crop, is renowned for its rich vegetable and medicinal values. However, the black-spot disease is relatively prevalent during its cultivation process, and there are no reports on using endophytes to control this disease. In this study, an endophytic bacterium that strongly antagonized <i>Alternaria alternata</i>, the pathogen causing yam black-spot disease, was isolated from ‘Tiegun’ yam leaves, and it was identified as a strain of <i>Bacillus</i> and named E-Do8. Further&#xa0;investigations revealed that the E-Do8 fermentation broth could significantly inhibit the spore germination and mycelial growth of <i>A. alternata</i>, and the mycelia showed uneven thickness, swelling, and irregular entanglement. Moreover, the ability of <i>A. alternata</i> to penetrate cellophane was weakened when cultured with the E-Do8 fermentation broth, which could also significantly inhibit the pathogenicity of <i>A. alternat</i>a. In addition, the UHPLC-Orbitrap Exploris 240 system was employed to analyze the active ingredients in the E-Do8 fermentation broth that inhibit <i>A. alternata</i>, and it was found that E-Do8 only produced surfactin-type compounds, C14-surfactin and surfactin. This study provided a theoretical foundation for the employment of <i>Dioscorea</i> endophytes in controlling its diseases.</p>

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Biocontrol of the black-spot disease pathogen (Alternaria alternata) in Dioscorea opposita by native endophytic bacterium Bacillus sp. E-Do8

  • 期刊论文

  • 发表日期：

    2025年6月22日

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  • Chaochuang Li,
  • Longlong Ma,
  • Houmin Wang,
  • Shunpeng Li,
  • Ruifei Wang,
  • Hao Zhang,
  • Mingjun Li,
  • Qingxiang Yang

摘要

Plant endophytic bacteria play crucial roles in the prevention and control of crop diseases. Yam (Dioscorea opposita), an important tuber and root crop, is renowned for its rich vegetable and medicinal values. However, the black-spot disease is relatively prevalent during its cultivation process, and there are no reports on using endophytes to control this disease. In this study, an endophytic bacterium that strongly antagonized Alternaria alternata, the pathogen causing yam black-spot disease, was isolated from ‘Tiegun’ yam leaves, and it was identified as a strain of Bacillus and named E-Do8. Further investigations revealed that the E-Do8 fermentation broth could significantly inhibit the spore germination and mycelial growth of A. alternata, and the mycelia showed uneven thickness, swelling, and irregular entanglement. Moreover, the ability of A. alternata to penetrate cellophane was weakened when cultured with the E-Do8 fermentation broth, which could also significantly inhibit the pathogenicity of A. alternata. In addition, the UHPLC-Orbitrap Exploris 240 system was employed to analyze the active ingredients in the E-Do8 fermentation broth that inhibit A. alternata, and it was found that E-Do8 only produced surfactin-type compounds, C14-surfactin and surfactin. This study provided a theoretical foundation for the employment of Dioscorea endophytes in controlling its diseases.