The effect of bioC gene on ultraviolet radiation and oxidative resistance of Bacillus thuringiensis (Bacillales: Bacillaceae)
摘要
Bacillus thuringiensis (Bacillales: Bacillaceae) (Bt) has played a significant role in pest control, which is the most intensively studied, rapidly developed, and widely used insecticidal microorganism in recent years. However, due to the influence of environmental stress, such as ultraviolet (UV) radiation and oxidative stress, its persistence is limited. Therefore, improving the stress resistance of Bt strains would be effective in prolonging the durability of related Bt products. Biotin is an important coenzyme that has been linked to stress responses in a variety of species. In this study, we successfully knocked down the bioC gene involved in biotin synthesis to further explore its function on the stress resistance of Bt. The removal of the target gene resulted in a decrease in Bt biotin content and a reduction in its survival rate under UV/oxidative stress. At the same time, the removal of bioC gene could reduce antioxidase activities. All of these observations demonstrated that biotin was functional for Bt stress resistance. This study therefore provides a theoretical basis for enriching Bt strain resistance genes and developing new Bt preparations.