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<p>High-intensity interval training (HIIT) is capable of reversing many aging-related metabolic differences in the proteome, but studies using proteomics to investigate the mechanism of the effects of HIIT on hepatic metabolic function in aged rats have not been reported. In this study, we investigated the effects of 8&#xa0;months of HIIT on mitochondrial oxidative function, oxidative stress, and inflammation in the liver of aged rats, and further explored the possible mechanisms of the metabolic effects of HIIT in aged rats by proteomics. The results of the study revealed that HIIT improved liver morphology, enhanced mitochondrial oxidative function, decreased inflammation and apoptosis levels, increased intrahepatic antioxidant function and inhibited ferroptosis in aged rats. Proteomics showed that HIIT altered changes in glycine, serine and threonine metabolic pathways in the liver, and further use of targeted amino acid metabolomics revealed that HIIT markedly increased glycine and serine content in aged livers. In vitro cells demonstrated that exogenous glycine supplementation significantly enhanced the intracellular antioxidant capacity of oxidatively stressed hepatocytes, while decreasing the level of inflammatory factor expression and significantly inhibiting the occurrence of ferroptosis. Our findings suggest that the improvement of metabolic function in aged liver tissue by HIIT may be associated with elevated glycine content, and that glycine within aged livers elevated by HIIT may mediate the maintenance of metabolic homeostasis within liver tissue.</p>

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Proteomics-based mechanism of HIIT regulation of hepatic metabolism in aging female rats

  • 期刊论文

  • 发表日期：

    2025年11月20日

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  • Jiahan He,
  • Zhuangzhi Wang,
  • Yibo Qiao,
  • Chenkai Zhang,
  • Zhi Yu,
  • Pinshi Ni,
  • Zhengyang Zhao,
  • Jianmei Cui,
  • Fanghui Li

摘要

High-intensity interval training (HIIT) is capable of reversing many aging-related metabolic differences in the proteome, but studies using proteomics to investigate the mechanism of the effects of HIIT on hepatic metabolic function in aged rats have not been reported. In this study, we investigated the effects of 8 months of HIIT on mitochondrial oxidative function, oxidative stress, and inflammation in the liver of aged rats, and further explored the possible mechanisms of the metabolic effects of HIIT in aged rats by proteomics. The results of the study revealed that HIIT improved liver morphology, enhanced mitochondrial oxidative function, decreased inflammation and apoptosis levels, increased intrahepatic antioxidant function and inhibited ferroptosis in aged rats. Proteomics showed that HIIT altered changes in glycine, serine and threonine metabolic pathways in the liver, and further use of targeted amino acid metabolomics revealed that HIIT markedly increased glycine and serine content in aged livers. In vitro cells demonstrated that exogenous glycine supplementation significantly enhanced the intracellular antioxidant capacity of oxidatively stressed hepatocytes, while decreasing the level of inflammatory factor expression and significantly inhibiting the occurrence of ferroptosis. Our findings suggest that the improvement of metabolic function in aged liver tissue by HIIT may be associated with elevated glycine content, and that glycine within aged livers elevated by HIIT may mediate the maintenance of metabolic homeostasis within liver tissue.