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<p>This study explores a possible therapy for managing atherosclerosis through macrophage modulation induced by CXCL4 receptor activity. Three herbal compounds potentially blocking the CXCL4 receptor, <i>i.e.</i>, amygdalin, 6-shogaol, and resveratrol, were tested <i>in silico</i> and <i>in vitro</i>. <i>In silico</i> analysis revealed the highest binding affinity of amygdalin to the CXCL4 receptor. Flow cytometry of peripheral blood mononuclear cells from 11 healthy subjects showed CD36 expression in groups treated with amygdalin, which attested to macrophage differentiation and maturation. ELISA showed increasing TNF-α production associated with enhanced cell phagocytosis, while MMP7 expression decreased, indicating lower molecular adhesion capability. Thus, amygdalin can potentially prevent M4 macrophage development, which plays a role in atherosclerotic plaque instability, though further validation is needed,</p>

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Effects of Amygdalin, 6-Shogaol, and Resveratrol on Inhibition of Differentiation of Monocyte-Derived M4 Macrophages: In Silico and In Vitro Studies

  • 期刊论文

  • 发表日期：

    2026年2月1日

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  • Mahdaleny,
  • Arleni Bustami,
  • Fadilah,
  • Febriana Catur Iswanti

摘要

This study explores a possible therapy for managing atherosclerosis through macrophage modulation induced by CXCL4 receptor activity. Three herbal compounds potentially blocking the CXCL4 receptor, i.e., amygdalin, 6-shogaol, and resveratrol, were tested in silico and in vitro. In silico analysis revealed the highest binding affinity of amygdalin to the CXCL4 receptor. Flow cytometry of peripheral blood mononuclear cells from 11 healthy subjects showed CD36 expression in groups treated with amygdalin, which attested to macrophage differentiation and maturation. ELISA showed increasing TNF-α production associated with enhanced cell phagocytosis, while MMP7 expression decreased, indicating lower molecular adhesion capability. Thus, amygdalin can potentially prevent M4 macrophage development, which plays a role in atherosclerotic plaque instability, though further validation is needed,