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<p>The article investigates the influence of extracellular vesicles (EVs) isolated from donor seminal plasma (SP) on the expression of key reproductive genes <i>AURKA</i> and <i>KLHL10</i> in human spermatozoa before and after cryopreservation. This study arises from the necessity to improve the effectiveness of assisted reproductive technologies (ART) and to advance the comprehension of how cryopreservation affects male gamete quality. A total of 17 sperm samples were examined. EVs were isolated from SP by asymmetric depth filtration and used for co-culturing with sperm samples prior to cryopreservation. Gene expression was analyzed by real-time PCR. The results demonstrated that sperm co-culturing with donor SP-EVs helped maintain or upregulate the expression of <i>AURKA</i> and <i>KLHL10</i> genes that play critical roles in cell division and acrosome formation, respectively. These findings indicate a protective effect of EVs on spermatozoa during cryopreservation. The study highlights the potential utility of SP-EVs for augmenting sperm cryotolerance and boosting the efficacy of ART programs. Further research is warranted to investigate protein-level changes and functional implications of the observed gene expression patterns.</p>

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Effect of the Vesicular Fraction of Donor Semen Plasma on the Expression of AURKA and KLHL10 Genes in Cryopreserved Spermatozoa

  • 期刊论文

  • 发表日期：

    2026年2月1日

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  • M. Yu. Gavrilov,
  • N. P. Makarova,
  • A. P. Sysoeva,
  • V. A. Zhuchkov,
  • E. A. Evtushenko,
  • E. E. Bragina,
  • V. S. Chernyshev,
  • E. A. Kalinina

摘要

The article investigates the influence of extracellular vesicles (EVs) isolated from donor seminal plasma (SP) on the expression of key reproductive genes AURKA and KLHL10 in human spermatozoa before and after cryopreservation. This study arises from the necessity to improve the effectiveness of assisted reproductive technologies (ART) and to advance the comprehension of how cryopreservation affects male gamete quality. A total of 17 sperm samples were examined. EVs were isolated from SP by asymmetric depth filtration and used for co-culturing with sperm samples prior to cryopreservation. Gene expression was analyzed by real-time PCR. The results demonstrated that sperm co-culturing with donor SP-EVs helped maintain or upregulate the expression of AURKA and KLHL10 genes that play critical roles in cell division and acrosome formation, respectively. These findings indicate a protective effect of EVs on spermatozoa during cryopreservation. The study highlights the potential utility of SP-EVs for augmenting sperm cryotolerance and boosting the efficacy of ART programs. Further research is warranted to investigate protein-level changes and functional implications of the observed gene expression patterns.