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<p>Hypergravity (HG) and microgravity (MG) modulate Ca<sup>2+</sup> entry into the cytosol of cardiomyocytes via both voltage-gated and mechanically gated Ca<sup>2+</sup> channels. The last include members of the TRP channel family with TRPM7 being the predominant subtype. We investigated the relationship between changes in TRPM7 channel protein synthesis and the abundance of its corresponding mRNA in cardiomyocytes from rats exposed to HG or MG for 14 days. Using transcriptome sequencing with normalization of “raw reads” by the TPM (Transcripts Per Kilobase Million) method, we found that TRPM7 TPM values significantly increased after HG exposure and significantly decreased after MG. Western blotting of TRPM7 protein levels confirmed a pronounced upregulation under HG and a substantial downregulation under MG. These changes may lead to altered ion conductance through mechanically gated channels in cardiomyocytes.</p>

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Hypergravity Upregulates and Simulated Microgravity Downregulates TRPM7 Mechanosensitive Ion Channel Expression in Isolated Rat Cardiomyocytes

  • 期刊论文

  • 发表日期：

    2025年11月1日

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  • A. D. Zolotareva,
  • V. I. Zolotarev,
  • O. V. Kamkina,
  • S. A. Shileiko,
  • V. M. Mitrokhin,
  • A. S. Bilichenko,
  • V. E. Kazansky,
  • A. S. Rodina,
  • M. I. Mladenov,
  • A. G. Kamkin

摘要

Hypergravity (HG) and microgravity (MG) modulate Ca2+ entry into the cytosol of cardiomyocytes via both voltage-gated and mechanically gated Ca2+ channels. The last include members of the TRP channel family with TRPM7 being the predominant subtype. We investigated the relationship between changes in TRPM7 channel protein synthesis and the abundance of its corresponding mRNA in cardiomyocytes from rats exposed to HG or MG for 14 days. Using transcriptome sequencing with normalization of “raw reads” by the TPM (Transcripts Per Kilobase Million) method, we found that TRPM7 TPM values significantly increased after HG exposure and significantly decreased after MG. Western blotting of TRPM7 protein levels confirmed a pronounced upregulation under HG and a substantial downregulation under MG. These changes may lead to altered ion conductance through mechanically gated channels in cardiomyocytes.