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<p>The release of cytochrome C into the cytoplasm after formation of a complex with mitochondrial phospholipids underlays the mitochondrial pathway of apoptosis. Changes in the structure of cytochrome C complex active center depending on phosphatidic acid concentration and at different temperatures (25, 37, and 45°C) were studied. Changes in the active center structure were assessed by optical absorption at 695 nm and compared with the intensity of luminol-dependent chemiluminescence which is proportional to the peroxidase activity of cytochrome C–phosphatidic acid complex. It was found that increasing the temperature from 25 to 45°C leads to comparable changes in the conformation of cytochrome C active center and an increase in its peroxidase activity.</p>

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Changes in the Structure of Cytochrome C Active Center under the Action of Phosphatidic Acid and Temperature

  • 期刊论文

  • 发表日期：

    2025年7月1日

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  • I. V. Kirilina,
  • G. O. Stepanov,
  • Yu. A. Vladimirov,
  • A. N. Osipov

摘要

The release of cytochrome C into the cytoplasm after formation of a complex with mitochondrial phospholipids underlays the mitochondrial pathway of apoptosis. Changes in the structure of cytochrome C complex active center depending on phosphatidic acid concentration and at different temperatures (25, 37, and 45°C) were studied. Changes in the active center structure were assessed by optical absorption at 695 nm and compared with the intensity of luminol-dependent chemiluminescence which is proportional to the peroxidase activity of cytochrome C–phosphatidic acid complex. It was found that increasing the temperature from 25 to 45°C leads to comparable changes in the conformation of cytochrome C active center and an increase in its peroxidase activity.