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<p>Progesterone receptors are involved in the regulation of tumor cell proliferation and therefore can be considered as a target of antitumor drugs. However, the question of the concordance of quantitative indicators of the protein and mRNA produced as a result of the expression of a single gene remains open. In this study, the concordance of the expression of the progesterone receptor protein and mRNA was evaluated using real-time PCR and flow cytometry, respectively, in 10 tumor cell lines (T47D, A375, PANC-1, SNU-1, HT-29, OVCAR-3, BXPC-3, HCC1954, A549, MCF-7). The expression of the progesterone receptor protein was detected in all studied cell lines; the expression of progesterone receptor mRNA was detected only in the T47D, which shows the discordance of these indicators and queries the relevance of measuring the expression of tumor marker mRNA when selecting the targeted anti-tumor drugs for the treatment of oncological diseases.</p>

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Comparative Analysis of Quantitative Indicators of Progesterone Receptor Protein and mRNA Expression in Tumor Cell Lines

  • 期刊论文

  • 发表日期：

    2025年5月1日

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  • I. P. Romanov,
  • T. A. Bogush,
  • A. A. Alimov,
  • A. Y. Kuzevanova,
  • A. M. Scherbakov,
  • V. S. Kosorukov

摘要

Progesterone receptors are involved in the regulation of tumor cell proliferation and therefore can be considered as a target of antitumor drugs. However, the question of the concordance of quantitative indicators of the protein and mRNA produced as a result of the expression of a single gene remains open. In this study, the concordance of the expression of the progesterone receptor protein and mRNA was evaluated using real-time PCR and flow cytometry, respectively, in 10 tumor cell lines (T47D, A375, PANC-1, SNU-1, HT-29, OVCAR-3, BXPC-3, HCC1954, A549, MCF-7). The expression of the progesterone receptor protein was detected in all studied cell lines; the expression of progesterone receptor mRNA was detected only in the T47D, which shows the discordance of these indicators and queries the relevance of measuring the expression of tumor marker mRNA when selecting the targeted anti-tumor drugs for the treatment of oncological diseases.