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<p>Researchers attribute increased incidence of colorectal cancer to environmental pollution with endocrine disruptors, including the estrogen-like substance bisphenol A (BPA). The proliferative activity, viability, and mRNA expression levels of the <i>PIK3CA</i>, <i>NFkBp65</i>, <i>VEGFA</i>, <i>ESR1</i>, <i>HIF1a</i>, <i>PTEN</i>, <i>TP53</i>, <i>CD44</i>, and <i>CDH1</i> genes were evaluated in human colon adenocarcinoma HT-29 cell culture incubated with different concentrations of BPA (5, 25, and 50 μM) for 24 and 96 h. Short-term (24 h) incubation with BPA did not lead to pronounced biological effects. During prolonged incubation (96 h), 5 μM BPA decreased <i>VEGFA</i> mRNA expression and increased <i>HIF1a</i> mRNA expression, 25 μM BPA increased the expression of <i>PIK3CA</i>, <i>ESR1</i>, and <i>HIF1a</i> mRNAs and decreased <i>VEGFA</i> and <i>CD44</i>, and 50 μM BPA increased the expression of <i>ESR1</i> mRNA and was cytotoxic. Hence, the duration of incubation and the concentration of BPA in the culture medium affect the progression of the colon cancer and its malignancy.</p>

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The Influence of Bisphenol A on the Molecular Biological Characteristics of Human Colon Adenocarcinoma HT-29 Culture Cells

  • 期刊论文

  • 发表日期：

    2025年5月1日

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  • E. A. Miroshnichenko,
  • M. A. Diatroptova,
  • A. I. Alekseeva,
  • A. D. Gerasimov,
  • A. M. Kosyreva

摘要

Researchers attribute increased incidence of colorectal cancer to environmental pollution with endocrine disruptors, including the estrogen-like substance bisphenol A (BPA). The proliferative activity, viability, and mRNA expression levels of the PIK3CA, NFkBp65, VEGFA, ESR1, HIF1a, PTEN, TP53, CD44, and CDH1 genes were evaluated in human colon adenocarcinoma HT-29 cell culture incubated with different concentrations of BPA (5, 25, and 50 μM) for 24 and 96 h. Short-term (24 h) incubation with BPA did not lead to pronounced biological effects. During prolonged incubation (96 h), 5 μM BPA decreased VEGFA mRNA expression and increased HIF1a mRNA expression, 25 μM BPA increased the expression of PIK3CA, ESR1, and HIF1a mRNAs and decreased VEGFA and CD44, and 50 μM BPA increased the expression of ESR1 mRNA and was cytotoxic. Hence, the duration of incubation and the concentration of BPA in the culture medium affect the progression of the colon cancer and its malignancy.