Approbation of a Homologous Model of the Antitumor Vaccine Based on Mature Mouse Dendritic Cells to Study the Biodistribution of the Cell Product

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发表日期：

2024年8月1日

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T. L. Nekhaeva,
I. D. Laskov,
E. I. Fedoros,
A. B. Danilova,
M. N. Yurova,
M. L. Tyndyk,
E. D. Ermakova,
N. V. Emelyanova,
N. A. Efremova,
A. V. Grigorevskaya,
M. A. Nekrasova,
I. A. Baldueva

摘要

Homologous animal cell product was obtained in protocol developed for female BALB/c mice. Dendritic cell (DC) migration from the injection site into the draining lymph nodes was evaluated. The number of DC labeled with carboxyfluorescein succinimidyl ester (CFSE) in draining lymph nodes increased from 5.3% (16 h) to 13.3% (48 h) (p=0.028) with a maximum at 72 h (15.4%, p=0.003). The immunophenotype of CFSE-DC detected in murine lymph nodes corresponded to the immunophenotype of mature vaccine DCs: they expressed differentiation markers CD11c, CD80, CD83, and CD86 (p>0.05 vs initial DC).