Comparative Morphological and Molecular Genetic Characteristics of Cell and Tissue Strains of Experimental Rat Glioma 10-17-2 (Astrid-17)

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发表日期：

2024年5月1日

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A. I. Alekseeva,
V. V. Kudelkina,
A. S. Khalansky,
A. V. Sentyabreva,
E. A. Miroshnichenko,
M. V. Gulyaev,
K. A. Rakitina,
A. M. Kosyreva

摘要

In order to obtain models of gliomas of varying degrees of malignancy, we performed morphological and molecular genetic study of a tissue strain of glioma 10-17-2 (Astrid-17) obtained by intracranial passaging of tumor fragments of chemically induced rat brain tumor, and a cell strain isolated from it. More or less pronounced changes in the expression levels of Mki67, Trp53, Vegfa, and Gfap genes in the tissue and cell strain of glioma 10-17-2 (Astrid-17) compared with intact brain tissue were shown. The tissue model of glioma 10-17-2 (Astrid-17) according to the studied characteristics shows features of grade 3-4 astrocytoma and the cellular model — grade 2-3 astrocytoma.