Delivery of Experimental mRNA Vaccine Encoding the RBD of SARS-CoV-2 by Jet Injection

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发表日期：

2024年4月1日

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D. N. Kisakov,
L. A. Kisakova,
S. V. Sharabrin,
V. A. Yakovlev,
E. V. Tigeeva,
M. B. Borgoyakova,
E. V. Starostina,
A. V. Zaikovskaya,
A. P. Rudometov,
N. B. Rudometova,
L. I. Karpenko,
A. A. Ilyichev

摘要

We studied a needle-free jet injection delivery of an experimental mRNA vaccine encoding the receptor-binding domain of the SARS-CoV-2 S protein (mRNA-RBD). Immunization of BALB/c mice with mRNA-RBD by a needle-free jet injector induced high levels of antibodies with virus-neutralizing activity and a virus-specific T-cell response. The immune response was low in the group of mice that received intramuscular injection of mRNA-RBD. The effectiveness of this simple and safe method of mRNA delivering has been demonstrated. Thus, jet injection of mRNA vaccine can be a good alternative to lipid nanoparticles.