Scholarlink

<p>Carbendazim (CBZ) is a fungicide that pollutes water bodies and has a detrimental effect on fish survival and health. This research investigated the effect of prolonged exposure to CBZ on the growth, survival, digestive functions, intestinal histology, and gene expression of <i>Oreochromis niloticus</i> (Nile tilapia). Furthermore, the restoration potential of dietary <i>Artemisia annua</i> (ART) against CBZ exposure was studied. Nile tilapia (<i>n</i> = 200; weight = 15.78 ± 0.10 g) were assigned to five treatments, each with four replicates (40 fish/treatment; 10 fish/replicate) for 60 days. The control and vehicle control groups were fed a basal diet and exposed to 0 and 0.216 µL/L dimethyl sulfoxide (DMSO), respectively. The ART group was fed a basal diet supplemented with 0.5% ART and was not exposed to CBZ. The CBZ and ART + CBZ groups were exposed to a nominal concentration of 2.16 µg/L CBZ and fed basal diets containing 0 and 0.5% ART, respectively. CBZ exposure decreased the growth performance and increased the feed conversion ratio. CBZ increased serum leptin levels, while lowering the intestinal activities of amylase, lipase, and trypsin. Oxidative stress was induced in the intestine by the inhibition of antioxidant enzymes (superoxide dismutase and glutathione peroxidase), along with a decline in total antioxidant capacity and an elevation of malondialdehyde following CBZ exposure. CBZ induced histological changes (necrosis and leukocytic infiltration) in the intestine and reduced morphometric measures (mucosal fold height and surface mucosal area). CBZ suppressed the intestinal expression of the tight junction protein (<i>zo-1</i>) and ion transporters (<i>slc26a6</i> and <i>nka</i>), while stimulating the expression of inflammatory (nuclear factor kappa-beta and interleukin-1β) and apoptotic (caspase-3) genes. Dietary ART (0.5%) improved fish growth and survival during CBZ exposure. ART application improved the intestinal antioxidant capacity and reduced the histological changes during CBZ exposure. In addition, the ART dietary intervention upregulated the intestinal expression of the tight junction protein and ion transporters and downregulated the inflammatory and apoptotic gene expression. Overall, dietary ART may help mitigate some adverse effects of nominal CBZ exposure on Nile tilapia growth and health under the present experimental conditions.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Efficacy of dietary Artemisia annua against chronic carbendazim exposure in Nile tilapia: growth, antioxidant capacity, intestinal morphology, inflammatory/apoptotic genes

  • 期刊论文

  • 发表日期：

    2026年7月4日

查看全文
  • Rowida E. Ibrahim,
  • Abdallah Tageldein Mansour,
  • Ahmed Saud Alsaqufi,
  • Yousef Ahmed Alkhamis,
  • Mohamed Ashour,
  • Roshmon Thomas Mathew,
  • Tarek Khamis,
  • Afaf N. Abdel Rahman,
  • Abdelhakeem El-Murr,
  • Mai A. M. El-son

摘要

Carbendazim (CBZ) is a fungicide that pollutes water bodies and has a detrimental effect on fish survival and health. This research investigated the effect of prolonged exposure to CBZ on the growth, survival, digestive functions, intestinal histology, and gene expression of Oreochromis niloticus (Nile tilapia). Furthermore, the restoration potential of dietary Artemisia annua (ART) against CBZ exposure was studied. Nile tilapia (n = 200; weight = 15.78 ± 0.10 g) were assigned to five treatments, each with four replicates (40 fish/treatment; 10 fish/replicate) for 60 days. The control and vehicle control groups were fed a basal diet and exposed to 0 and 0.216 µL/L dimethyl sulfoxide (DMSO), respectively. The ART group was fed a basal diet supplemented with 0.5% ART and was not exposed to CBZ. The CBZ and ART + CBZ groups were exposed to a nominal concentration of 2.16 µg/L CBZ and fed basal diets containing 0 and 0.5% ART, respectively. CBZ exposure decreased the growth performance and increased the feed conversion ratio. CBZ increased serum leptin levels, while lowering the intestinal activities of amylase, lipase, and trypsin. Oxidative stress was induced in the intestine by the inhibition of antioxidant enzymes (superoxide dismutase and glutathione peroxidase), along with a decline in total antioxidant capacity and an elevation of malondialdehyde following CBZ exposure. CBZ induced histological changes (necrosis and leukocytic infiltration) in the intestine and reduced morphometric measures (mucosal fold height and surface mucosal area). CBZ suppressed the intestinal expression of the tight junction protein (zo-1) and ion transporters (slc26a6 and nka), while stimulating the expression of inflammatory (nuclear factor kappa-beta and interleukin-1β) and apoptotic (caspase-3) genes. Dietary ART (0.5%) improved fish growth and survival during CBZ exposure. ART application improved the intestinal antioxidant capacity and reduced the histological changes during CBZ exposure. In addition, the ART dietary intervention upregulated the intestinal expression of the tight junction protein and ion transporters and downregulated the inflammatory and apoptotic gene expression. Overall, dietary ART may help mitigate some adverse effects of nominal CBZ exposure on Nile tilapia growth and health under the present experimental conditions.