Scholarlink

<p>The expansion of saline-alkaline waters worldwide is impacting aquaculture space, creating an urgent need to expand the range of fish species suitable for cultivation in such environments. Although grass carp (<i>Ctenopharyngodon idella</i>) exhibits a certain degree of tolerance to alkalinity, the underlying molecular regulatory mechanisms remain unclear. In this study, a 30-day chronic exposure experiment was conducted, with grass carp exposed to three alkalinity levels: control (A0, 0&#xa0;mmol/L) and two stress groups (A10, 10&#xa0;mmol/L; A20, 20&#xa0;mmol/L). The biochemical and transcriptomic changes in the intestine were investigated to comprehensively understand the effects of low and moderate alkalinity on grass carp and explore the regulatory mechanisms of alkalinity tolerance. Throughout the experiment, no mortality or oxidative damage was observed. Transcriptomic analysis revealed 1020, 1040, and 1332 DEGs in the A10 vs A0, A20 vs A0, and A20 vs A10 comparison groups, respectively. Transcriptomic analysis revealed that lipid metabolism was modulated by the activation of the PPAR signaling pathway and primary bile acid biosynthesis in grass carp, thereby ensuring energy supply and nutrient absorption under alkaline conditions. Glutathione metabolism is activated to counteract oxidative stress. This study demonstrated that grass carp can adapt to low and moderate-alkalinity (A10 and A20) environments by regulating biochemical and metabolic pathways, reflecting its tolerance to alkalinity stress. These findings enhance our understanding of the mechanisms underlying the response of grass carp to alkaline stress and provide a theoretical basis for grass carp cultivation in moderately alkaline waters.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Intestinal transcriptomic responses to chronic carbonate alkalinity stress in grass carp (Ctenopharyngodon idella)

  • 期刊论文

  • 发表日期：

    2026年6月29日

查看全文
  • Feng Li,
  • Tianqi Liu,
  • Kexin Zhang,
  • Zhipeng Sun,
  • Rongbin Na,
  • Yumei Chang,
  • Cuiyun Lu,
  • Xianhu Zheng

摘要

The expansion of saline-alkaline waters worldwide is impacting aquaculture space, creating an urgent need to expand the range of fish species suitable for cultivation in such environments. Although grass carp (Ctenopharyngodon idella) exhibits a certain degree of tolerance to alkalinity, the underlying molecular regulatory mechanisms remain unclear. In this study, a 30-day chronic exposure experiment was conducted, with grass carp exposed to three alkalinity levels: control (A0, 0 mmol/L) and two stress groups (A10, 10 mmol/L; A20, 20 mmol/L). The biochemical and transcriptomic changes in the intestine were investigated to comprehensively understand the effects of low and moderate alkalinity on grass carp and explore the regulatory mechanisms of alkalinity tolerance. Throughout the experiment, no mortality or oxidative damage was observed. Transcriptomic analysis revealed 1020, 1040, and 1332 DEGs in the A10 vs A0, A20 vs A0, and A20 vs A10 comparison groups, respectively. Transcriptomic analysis revealed that lipid metabolism was modulated by the activation of the PPAR signaling pathway and primary bile acid biosynthesis in grass carp, thereby ensuring energy supply and nutrient absorption under alkaline conditions. Glutathione metabolism is activated to counteract oxidative stress. This study demonstrated that grass carp can adapt to low and moderate-alkalinity (A10 and A20) environments by regulating biochemical and metabolic pathways, reflecting its tolerance to alkalinity stress. These findings enhance our understanding of the mechanisms underlying the response of grass carp to alkaline stress and provide a theoretical basis for grass carp cultivation in moderately alkaline waters.