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<p>Low temperature exposure during the winter season considerably retards the growth and survival in rohu, <i>Labeo rohita</i>. Low temperature exposure has a profound effect on digestion and metabolism and may lead to metabolic shifting. Hence, a sixty days experiment was conducted to understand the carbohydrate digestion and utilization in herbivorous fish, <i>L. rohita</i> fingerlings exposed to low temperature (18.20 ± 0.15 °C) in RAS (Re-circulatory Aquaculture System) attached with an online chiller to mimic the winter conditions. 120 <i>L. rohita</i> fingerlings (average weight 9.23 ± 0.13&#xa0;g) were stocked randomly into four different groups and fed with four semi-purified diets comprising 90—g lipid and 300—g crude protein/kg diet with graded levels of carbohydrates (nitrogen free extract). Accordingly, the experimental groups were C37, C40, C43 and C43 (370, 400, 430, 460&#xa0;g carbohydrate/kg diet). The best growth performance was seen in the C40 fed group, whereas the same group exhibited a significantly lower feed conversion ratio (<i>P</i> &lt; 0.05). The digestive enzymes activities were significantly (<i>P</i> &lt; 0.05) increased with the increasing dietary carbohydrate levels. The hexokinase activity also increased with the increasing dietary carbohydrate levels, but pyruvate kinase (PFK), glucose-6-phosphate dehydrogenase (G6PD) and isocitrate dehydrogenase (IDH) enzyme activities were significantly higher in C40 group fish. The SOD and catalase activities were significantly (<i>P</i> &lt; 0.05) low at lower carbohydrate levels (C37 and C40 groups). The serum albumin and globulin levels were significantly higher (<i>P</i> &lt; 0.05) in C40 group fish. The gene expression study confirmed that C40 group fish had shown better carbohydrate utility. The findings of this study suggest that <i>L. rohita</i> fish fingerling can utilize dietary carbohydrate efficiently up to 400&#xa0;g/kg diet when reared under low temperature mimicking the winter conditions.</p>

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Impact of low temperature on carbohydrate utilization, enzymes activities and gene expression in rohu (Labeo rohita) fingerlings

  • 期刊论文

  • 发表日期：

    2026年5月16日

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  • Shivkumar Swamy,
  • Ashutosh D. Deo,
  • Manish Jayant,
  • Narottam P. Sahu,
  • Naseemashahul Shamna,
  • Kiran D. Rasal,
  • Dhanalakshmi M.,
  • Husain Nottanalan

摘要

Low temperature exposure during the winter season considerably retards the growth and survival in rohu, Labeo rohita. Low temperature exposure has a profound effect on digestion and metabolism and may lead to metabolic shifting. Hence, a sixty days experiment was conducted to understand the carbohydrate digestion and utilization in herbivorous fish, L. rohita fingerlings exposed to low temperature (18.20 ± 0.15 °C) in RAS (Re-circulatory Aquaculture System) attached with an online chiller to mimic the winter conditions. 120 L. rohita fingerlings (average weight 9.23 ± 0.13 g) were stocked randomly into four different groups and fed with four semi-purified diets comprising 90—g lipid and 300—g crude protein/kg diet with graded levels of carbohydrates (nitrogen free extract). Accordingly, the experimental groups were C37, C40, C43 and C43 (370, 400, 430, 460 g carbohydrate/kg diet). The best growth performance was seen in the C40 fed group, whereas the same group exhibited a significantly lower feed conversion ratio (P < 0.05). The digestive enzymes activities were significantly (P < 0.05) increased with the increasing dietary carbohydrate levels. The hexokinase activity also increased with the increasing dietary carbohydrate levels, but pyruvate kinase (PFK), glucose-6-phosphate dehydrogenase (G6PD) and isocitrate dehydrogenase (IDH) enzyme activities were significantly higher in C40 group fish. The SOD and catalase activities were significantly (P < 0.05) low at lower carbohydrate levels (C37 and C40 groups). The serum albumin and globulin levels were significantly higher (P < 0.05) in C40 group fish. The gene expression study confirmed that C40 group fish had shown better carbohydrate utility. The findings of this study suggest that L. rohita fish fingerling can utilize dietary carbohydrate efficiently up to 400 g/kg diet when reared under low temperature mimicking the winter conditions.