<p>High-density aquaculture of the American bullfrog (<i>Aquarana catesbeiana</i>) frequently results in hepatic lipid accumulation and intestinal damage. The aim of this study was to determine whether dietary glycyrrhizic acid (GA) can improve growth performance and hepato-intestinal health of bullfrogs reared under high-density conditions, and to identify an effective inclusion level. A total of 1500 bullfrogs were randomly assigned to five dietary groups, receiving diets supplemented with 0 g/kg (GA0), 0.3 g/kg (GA1), 1.5 g/kg (GA2), 3 g/kg (GA3), and 5 g/kg (GA4) GA over a 35-day period. Our findings showed that GA supplementation significantly enhanced the weight gain and survival rates of bullfrogs compared with GA0 (<i>P</i> &lt; 0.05), while exerting beneficial effects on their hepato-intestinal health. In the intestine, GA improved morphology and increased barrier function, as indicated by increased villus height, goblet cell density, and upregulated tight junction–related gene expression (<i>zo-1</i>, <i>zo-2</i>, <i>zo-3</i>, <i>occludin</i>, and <i>claudin-7</i>). In the liver, GA bolstered antioxidant capacity, evidenced by elevated activities of the key antioxidant enzymes SOD, CAT, and GSH-Px, along with a reduced level of the lipid peroxidation product malondialdehyde (MDA) relative to GA0 (<i>P</i> &lt; 0.05). Histological assessments also showed that GA supplementation diminished the number and size of hepatic lipid droplets, effectively mitigating hepatic lipid deposition. Molecularly, this corresponded with downregulated lipogenic genes (<i>acc1</i>, <i>pparγ</i>) and the upregulated lipolytic gene (<i>atgl</i>) (<i>P</i> &lt; 0.05). Notably, these positive effects generally increased with GA levels up to a peak at 1.5 g/kg (GA2) before declining. This research offers a scientific foundation for utilizing GA as a functional feed additive in bullfrog aquaculture, potentially improving growth, feed efficiency, and overall health.</p>