Scholarlink

<p>Probiotics improve immunity and reduce dependence on antibiotics, supporting sustainable aquaculture. Currently available commercial probiotics originate from non-aquatic environments; however, their efficacy in aquaculture remains limited. This study aimed to identify indigenous probiotics from the midguts of gibel carp (<i>Carassius auratus gibelio</i>) as effective and host-specific probiotics. Using agar culture plates, we isolated 112 bacterial isolates (47 from farmed carp and 65 from wild carp). Specifically, enzyme production activity assays in culture indicated that isolates SY01 and SY02 exhibited prominent production of amylase, protease, and cellulase, and were non-pathogenic to gibel carp. Morphological and genome sequence analyses identified these isolates as <i>Bacillus subtilis</i> and <i>Bacillus amyloliquefaciens</i>, respectively. Supplementation of gibel carp diets with 1 × 10⁷ CFU/g⋅diet for 30&#xa0;days significantly improved the growth performance, digestive enzyme activity, and serum antioxidant capacity (superoxide dismutase, total antioxidant capacity, and catalase). Supplementation also upregulated the expression of immune-related genes, such as intelectin 1, interleukin 11, and interferon. Additionally, when the fish were intraperitoneally challenged by cyprinid herpesvirus 2 (CyHV-2), supplementation significantly increased the fish survival rate from 20.8% (control) to 66.7% (SY01) and 54.2% (SY02). The two host-derived probiotics improved the growth, digestion, antioxidant status, and disease resistance in gibel carp, revealing their potential as effective substitutes for antibiotics. These findings support the potential application of indigenous probiotics to advance sustainable and environmentally friendly aquaculture practices.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Evaluation of novel probiotic Bacillus candidates from the midgut of gibel carp (Carassius auratus gibelio): effects on fish growth, immunity, and resistance to CyHV-2 infection

  • 期刊论文

  • 发表日期：

    2026年3月3日

查看全文
  • Mingming Zhang,
  • Ni Ma,
  • Yaqin Yang,
  • Mingfeng Zhu,
  • Hu Zhang,
  • Zhigang Zhao,
  • Jialin Zhang,
  • Mingzhu Pan,
  • Zhitao Qi,
  • Guo Qiao

摘要

Probiotics improve immunity and reduce dependence on antibiotics, supporting sustainable aquaculture. Currently available commercial probiotics originate from non-aquatic environments; however, their efficacy in aquaculture remains limited. This study aimed to identify indigenous probiotics from the midguts of gibel carp (Carassius auratus gibelio) as effective and host-specific probiotics. Using agar culture plates, we isolated 112 bacterial isolates (47 from farmed carp and 65 from wild carp). Specifically, enzyme production activity assays in culture indicated that isolates SY01 and SY02 exhibited prominent production of amylase, protease, and cellulase, and were non-pathogenic to gibel carp. Morphological and genome sequence analyses identified these isolates as Bacillus subtilis and Bacillus amyloliquefaciens, respectively. Supplementation of gibel carp diets with 1 × 10⁷ CFU/g⋅diet for 30 days significantly improved the growth performance, digestive enzyme activity, and serum antioxidant capacity (superoxide dismutase, total antioxidant capacity, and catalase). Supplementation also upregulated the expression of immune-related genes, such as intelectin 1, interleukin 11, and interferon. Additionally, when the fish were intraperitoneally challenged by cyprinid herpesvirus 2 (CyHV-2), supplementation significantly increased the fish survival rate from 20.8% (control) to 66.7% (SY01) and 54.2% (SY02). The two host-derived probiotics improved the growth, digestion, antioxidant status, and disease resistance in gibel carp, revealing their potential as effective substitutes for antibiotics. These findings support the potential application of indigenous probiotics to advance sustainable and environmentally friendly aquaculture practices.