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<p>Developing a polyvalent vaccine strategy offers promising protection against diverse heterologous strains of <i>Edwardsiella ictaluri</i>. This study evaluated the efficacy and immune responses induced by an inactivated whole-cell polyvalent <i>E. ictaluri</i> vaccine, administered with or without a booster, in striped catfish (<i>Pangasianodon hypophthalmus</i>). Fish were vaccinated using inactivated polyvalent bacterin at concentrations of 10<sup>1</sup>⁰ and 10⁹ CFU mL⁻<sup>1</sup>. Vaccine performance was assessed through both specific and non-specific immune parameters and relative percent survival (RPS). The vaccine at 10<sup>1</sup>⁰ CFU mL⁻<sup>1</sup> with a booster significantly enhanced antibody titers, phagocytic and lysozyme activities, respiratory burst, lymphocyte counts, and the expression of <i>MHC class II</i> and <i>IL-1β</i> genes. This formulation achieved the highest RPS values of 90.47%, 55%, and 60% against pathogenic <i>E. ictaluri</i> isolates PJbH, P, and PBm1G, respectively. In contrast, non-booster groups exhibited lower protection (RPS 61.90%, 42.85%, and 44.64%). In conclusion, the inactivated polyvalent vaccine at 10<sup>1</sup>⁰ CFU mL⁻<sup>1</sup>, with a booster, effectively strengthened both humoral and cellular immunity, providing robust protection against <i>E. ictaluri</i> infection in striped catfish.</p>

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Efficacy of an inactivated polyvalent Edwardsiella ictaluri vaccine with booster in enhancing immune response and protection in striped catfish (Pangasianodon hypophthalmus)

  • 期刊论文

  • 发表日期：

    2026年1月15日

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  • Uni Purwaningsih,
  • Sukenda Sukenda,
  • Angela Mariana Lusiastuti,
  • Alimuddin Alimuddin,
  • Widanarni Widanarni,
  • Sri Nuryati,
  • Dendi Hidayatullah

摘要

Developing a polyvalent vaccine strategy offers promising protection against diverse heterologous strains of Edwardsiella ictaluri. This study evaluated the efficacy and immune responses induced by an inactivated whole-cell polyvalent E. ictaluri vaccine, administered with or without a booster, in striped catfish (Pangasianodon hypophthalmus). Fish were vaccinated using inactivated polyvalent bacterin at concentrations of 101⁰ and 10⁹ CFU mL⁻1. Vaccine performance was assessed through both specific and non-specific immune parameters and relative percent survival (RPS). The vaccine at 101⁰ CFU mL⁻1 with a booster significantly enhanced antibody titers, phagocytic and lysozyme activities, respiratory burst, lymphocyte counts, and the expression of MHC class II and IL-1β genes. This formulation achieved the highest RPS values of 90.47%, 55%, and 60% against pathogenic E. ictaluri isolates PJbH, P, and PBm1G, respectively. In contrast, non-booster groups exhibited lower protection (RPS 61.90%, 42.85%, and 44.64%). In conclusion, the inactivated polyvalent vaccine at 101⁰ CFU mL⁻1, with a booster, effectively strengthened both humoral and cellular immunity, providing robust protection against E. ictaluri infection in striped catfish.