Scholarlink

<p><i>Vibrio harveyi</i> is a critical pathogen in aquaculture, capable of inducing severe hepatic damage and metabolic disorders in fish. Comprehensive histopathological, biochemical, transcriptomic, and metabolomic analyses were performed to elucidate the impact of oligochitosan supplementation on hybrid grouper subjected to <i>Vibrio harveyi</i> infection. Growth performance analysis demonstrated that <i>V. harveyi</i> infection markedly elevated the hepatopancreas somatic index, whereas this was effectively attenuated by oligochitosan supplementation. Histopathological findings confirmed that oligochitosan supplementation attenuated <i>V. harveyi</i>-induced pancreatic lesions, including hypertrophy, vacuolization, and apoptosis. Biochemical evaluation showed that oligochitosan improved GOT and SOD activities and mitigated oxidative stress by lowering MDA content. Transcriptomic profiling revealed significant enrichment of immune and metabolic pathways, particularly Toll-like receptor signaling and amino acid degradation, as identified by KEGG analysis. qRT-PCR confirmation revealed that oligochitosan intervention attenuated inflammatory signaling by significantly decreasing the transcript levels of <i>IL-8</i> and <i>TLR-3</i>. Metabolomic analysis revealed substantial alterations in lipid and glycolipid-related metabolites, with KEGG enrichment indicating notable changes in lipid metabolism. Notably, integrated multi-omics correlation analysis revealed that immune-related genes, such as complement component 7b (<i>c7b</i>) and haptoglobin (<i>hp</i>), were positively associated with anti-inflammatory and lipid metabolism–related metabolites, whereas the lipid synthesis gene pancreatic progenitor cell differentiation and proliferation factor b (<i>ppdpfb</i>) showed a negative association with these metabolites. In summary, oligochitosan supplementation mitigated liver damage in hybrid grouper infected with <i>V. harveyi</i> and modulated immune and metabolic responses, highlighting its potential as a functional feed additive in aquaculture.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Influence of oligochitosan on hepatic immune-metabolic homeostasis in hybrid grouper infected with Vibrio harveyi

  • 期刊论文

  • 发表日期：

    2026年1月10日

查看全文
  • Lixin Ma,
  • Zhilong Chen,
  • Zhuojin He,
  • Xiaomin Zheng,
  • Jiawen Lin,
  • Jiayue Yin,
  • Cuiyun Zou,
  • Li Lin,
  • Fei Shi

摘要

Vibrio harveyi is a critical pathogen in aquaculture, capable of inducing severe hepatic damage and metabolic disorders in fish. Comprehensive histopathological, biochemical, transcriptomic, and metabolomic analyses were performed to elucidate the impact of oligochitosan supplementation on hybrid grouper subjected to Vibrio harveyi infection. Growth performance analysis demonstrated that V. harveyi infection markedly elevated the hepatopancreas somatic index, whereas this was effectively attenuated by oligochitosan supplementation. Histopathological findings confirmed that oligochitosan supplementation attenuated V. harveyi-induced pancreatic lesions, including hypertrophy, vacuolization, and apoptosis. Biochemical evaluation showed that oligochitosan improved GOT and SOD activities and mitigated oxidative stress by lowering MDA content. Transcriptomic profiling revealed significant enrichment of immune and metabolic pathways, particularly Toll-like receptor signaling and amino acid degradation, as identified by KEGG analysis. qRT-PCR confirmation revealed that oligochitosan intervention attenuated inflammatory signaling by significantly decreasing the transcript levels of IL-8 and TLR-3. Metabolomic analysis revealed substantial alterations in lipid and glycolipid-related metabolites, with KEGG enrichment indicating notable changes in lipid metabolism. Notably, integrated multi-omics correlation analysis revealed that immune-related genes, such as complement component 7b (c7b) and haptoglobin (hp), were positively associated with anti-inflammatory and lipid metabolism–related metabolites, whereas the lipid synthesis gene pancreatic progenitor cell differentiation and proliferation factor b (ppdpfb) showed a negative association with these metabolites. In summary, oligochitosan supplementation mitigated liver damage in hybrid grouper infected with V. harveyi and modulated immune and metabolic responses, highlighting its potential as a functional feed additive in aquaculture.