Scholarlink

<p>The present study highlights the first-ever report of β-defensin (TbBD) from the fish silver pompano, <i>Trachinotus blochii.</i> Gene sequence analysis revealed an open reading frame (ORF) of 189 nucleotides, encoding a peptide consisting of 63 amino acids with a molecular mass of 7.2&#xa0;kDa and a net charge of + 4.5. Phylogenetic analysis indicated a close association of TbBD with β-defensin from <i>Trachinotus ovatus</i>. In silico functional analysis predicted cell-penetrating, anticancer, antibacterial, antiviral, and pro-inflammatory properties, while non-toxicity and thermal stability suggest potential therapeutic applications. Real-time PCR analysis revealed TbBD expression across all developmental stages, peaking at 15&#xa0;days post-hatch, indicating a protective role during early larval development and continued involvement in host defense. Further scientific investigations into the antimicrobial efficacy of TbBD, either as a synthetic or recombinant peptide, could reveal its usefulness as a viable therapeutic agent in aquaculture and medicine.</p> Graphical abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Molecular characterization and antimicrobial prospects of a novel β-defensin from silver pompano (Trachinotus blochii)

  • 期刊论文

  • 发表日期：

    2025年12月9日

查看全文
  • Anne Maria Thomas,
  • K. L. Dhanya Lenin,
  • T. J. Greeshma,
  • K. K. Noorjahan,
  • Swapna P. Antony

摘要

The present study highlights the first-ever report of β-defensin (TbBD) from the fish silver pompano, Trachinotus blochii. Gene sequence analysis revealed an open reading frame (ORF) of 189 nucleotides, encoding a peptide consisting of 63 amino acids with a molecular mass of 7.2 kDa and a net charge of + 4.5. Phylogenetic analysis indicated a close association of TbBD with β-defensin from Trachinotus ovatus. In silico functional analysis predicted cell-penetrating, anticancer, antibacterial, antiviral, and pro-inflammatory properties, while non-toxicity and thermal stability suggest potential therapeutic applications. Real-time PCR analysis revealed TbBD expression across all developmental stages, peaking at 15 days post-hatch, indicating a protective role during early larval development and continued involvement in host defense. Further scientific investigations into the antimicrobial efficacy of TbBD, either as a synthetic or recombinant peptide, could reveal its usefulness as a viable therapeutic agent in aquaculture and medicine.

Graphical abstract