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<p>Reproduction in vertebrates is primarily regulated by the gonadotropin-releasing hormone (GnRH) neuropeptide, but limited knowledge of its molecular mechanisms in <i>Channa striata</i> (striped murrel) affects the development of effective breeding strategies. This study aims to identify coding sequences of GnRH 3 neuropeptide of <i>C. striata</i>, consisting of 303&#xa0;bp, encoding 66 amino acids, comprising a 7.26-kDa peptide, including a decapeptide, proteolytic sites, and species-specific GnRH-associated peptide. Molecular docking and dynamic simulation of csGnRH 3-csGnRHR depicted robust interaction (docking score − 4578.66). Further, the mature brooders of <i>C. striata</i> selected and injected with salmonGnRHa and gene expression analysis have been done at different time intervals, showing &gt; eightfold increase in GnRH expression in salmonGnRHa-treated females, while no significant expression was observed in males. In females, FSH and LH expression levels increased (<i>p</i> &lt; 0.05) significantly in the brain pituitary after 12&#xa0;h, while negligible expression was observed in males. Histology showed different oocyte stages in female gonads; males exhibited immature testes, confirming their asynchronous behavior. Hormonal analysis showed elevated progesterone (313 ± 8&#xa0;pg/mL), estradiol (349 ± 6&#xa0;pg/mL), and testosterone (129 ± 3&#xa0;pg/mL) in females at 6&#xa0;h, aiding ovulation. In males, testosterone (96 ± 8&#xa0;pg/mL) slightly increased at 6&#xa0;h, but remained low, showing asynchronous maturation in murrel. This work provided molecular and structural insights into csGnRH 3 neuropeptide and will help in development of a species-specific inducing agent for induced breeding.</p>

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Molecular insights into GnRH3-associated peptide structure and reproductive responses in Channa striata stimulated with sGnRH analogue

  • 期刊论文

  • 发表日期：

    2025年11月17日

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  • Prachi Asgolkar,
  • Kiran D. Rasal,
  • Rajesh Kumar,
  • Uday Udit,
  • Arvind Sonwane,
  • Manoj Brahmane,
  • Sunil Kumar,
  • Mayank Rashmi,
  • Girish Kumar Jha,
  • Jitendra Kumar Sundaray,
  • Megha Bedekar,
  • Mukunda Goswami

摘要

Reproduction in vertebrates is primarily regulated by the gonadotropin-releasing hormone (GnRH) neuropeptide, but limited knowledge of its molecular mechanisms in Channa striata (striped murrel) affects the development of effective breeding strategies. This study aims to identify coding sequences of GnRH 3 neuropeptide of C. striata, consisting of 303 bp, encoding 66 amino acids, comprising a 7.26-kDa peptide, including a decapeptide, proteolytic sites, and species-specific GnRH-associated peptide. Molecular docking and dynamic simulation of csGnRH 3-csGnRHR depicted robust interaction (docking score − 4578.66). Further, the mature brooders of C. striata selected and injected with salmonGnRHa and gene expression analysis have been done at different time intervals, showing > eightfold increase in GnRH expression in salmonGnRHa-treated females, while no significant expression was observed in males. In females, FSH and LH expression levels increased (p < 0.05) significantly in the brain pituitary after 12 h, while negligible expression was observed in males. Histology showed different oocyte stages in female gonads; males exhibited immature testes, confirming their asynchronous behavior. Hormonal analysis showed elevated progesterone (313 ± 8 pg/mL), estradiol (349 ± 6 pg/mL), and testosterone (129 ± 3 pg/mL) in females at 6 h, aiding ovulation. In males, testosterone (96 ± 8 pg/mL) slightly increased at 6 h, but remained low, showing asynchronous maturation in murrel. This work provided molecular and structural insights into csGnRH 3 neuropeptide and will help in development of a species-specific inducing agent for induced breeding.