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<p>This study investigated the effects of supplementing high soybean meal diets with alpha-ketoglutaric acid (AKG) on the intestinal health and growth performance of spotted sea bass (<i>Lateolabrax maculatus</i>). A total of 270 fish (average weight 7.48 ± 0.05&#xa0;g) were randomly divided into 6 groups (3 replicates each, 15 fish per tank): negative control (FM, 44% fish meal), positive control (HSB, 22% fish meal + 40% soybean meal), and four experimental groups fed the HSB supplemented with 0.5%, 1%, 1.5%, and 2% AKG, respectively. Fish were fed twice daily for 56&#xa0;days, with serum biochemical indices, foregut histology, and inflammatory gene expression detected post-experiment. The research results are as follows: Diets high in soybean meal impaired growth, as seen in significantly lower weight gain (WG), specific growth rate (SGR), biomass gain (BG), and daily feed intake (DFI) compared to the FM group; AKG supplementation did not alter these growth parameters significantly. Histological examination of the foregut revealed that fish fed the high soybean meal diet exhibited pronounced intestinal damage compared with those receiving the fish meal diet. This injury was accompanied by a marked upregulation of the pro-inflammatory cytokine tumor necrosis factor-α (TNF-α). However, dietary supplementation with 1.5% AKG mitigated these effects: TNF-α expression was significantly reduced, while the anti-inflammatory cytokine interleukin-10 (IL-10) was concurrently elevated, indicating a shift toward an anti-inflammatory milieu. In terms of serum biochemical indices, supplementation with 1.5% AKG significantly elevated the levels of complement 3 (C3), alkaline phosphatase (AKP), lysozyme (LZM), and glutathione (GSH) in the serum. The findings indicate that a high soybean meal diet causes intestinal damage and reduces the growth performance of spotted sea bass, while the supplementation of AKG fails to enhance their growth performance and has limited ability to repair intestinal damage.</p>

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Effects of dietary alpha-ketoglutaric acid on growth performance and intestinal barrier function in Lateolabrax maculatus fed a high soybean meal diet

  • 期刊论文

  • 发表日期：

    2025年11月18日

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  • Fuqiang Quan,
  • Jianrong Ma,
  • Yanbo Zhao,
  • Hao Lin,
  • ZhongBao Li

摘要

This study investigated the effects of supplementing high soybean meal diets with alpha-ketoglutaric acid (AKG) on the intestinal health and growth performance of spotted sea bass (Lateolabrax maculatus). A total of 270 fish (average weight 7.48 ± 0.05 g) were randomly divided into 6 groups (3 replicates each, 15 fish per tank): negative control (FM, 44% fish meal), positive control (HSB, 22% fish meal + 40% soybean meal), and four experimental groups fed the HSB supplemented with 0.5%, 1%, 1.5%, and 2% AKG, respectively. Fish were fed twice daily for 56 days, with serum biochemical indices, foregut histology, and inflammatory gene expression detected post-experiment. The research results are as follows: Diets high in soybean meal impaired growth, as seen in significantly lower weight gain (WG), specific growth rate (SGR), biomass gain (BG), and daily feed intake (DFI) compared to the FM group; AKG supplementation did not alter these growth parameters significantly. Histological examination of the foregut revealed that fish fed the high soybean meal diet exhibited pronounced intestinal damage compared with those receiving the fish meal diet. This injury was accompanied by a marked upregulation of the pro-inflammatory cytokine tumor necrosis factor-α (TNF-α). However, dietary supplementation with 1.5% AKG mitigated these effects: TNF-α expression was significantly reduced, while the anti-inflammatory cytokine interleukin-10 (IL-10) was concurrently elevated, indicating a shift toward an anti-inflammatory milieu. In terms of serum biochemical indices, supplementation with 1.5% AKG significantly elevated the levels of complement 3 (C3), alkaline phosphatase (AKP), lysozyme (LZM), and glutathione (GSH) in the serum. The findings indicate that a high soybean meal diet causes intestinal damage and reduces the growth performance of spotted sea bass, while the supplementation of AKG fails to enhance their growth performance and has limited ability to repair intestinal damage.