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<p><i>Dunaliella</i>, known for its high salt tolerance and photosynthetic efficiency, plays a significant role in industrial and food production. This study presents a comparative analysis of the lycopene biosynthesis pathway in <i>Dunaliella salina</i> and <i>Dunaliella bardawil</i>. Genetic material from both species was extracted and expressed in a bacterial system, followed by HPLC, molecular docking, and phylogenetic analysis. The all-<i>trans</i>-lycopene content in the DsCRT strain was 1.26 ± 0.03&#xa0;mg/g DCW, higher than that of the DbCRT strain. Molecular docking analysis showed that the binding affinity between DsCRTISO and the substrate was -11.18&#xa0;kcal/mol, which was lower than the binding affinity of DbCRTISO (-10.08&#xa0;kcal/mol), revealing that the catalytic activity of DsCRTISO was greater than that of DbCRTISO. Phylogenetic analysis confirmed a close relationship between <i>D. salina</i> and <i>D</i>. <i>bardawil</i>. HPLC showed that <i>D. bardawil</i> accumulate minimal β-carotene, likely due to the failure of specific gene regulatory mechanisms, despite sharing conserved CRTISO domains with <i>D. salina</i>. This study demonstrates that lycopene metabolism enzyme activity in <i>D. salina</i> is significantly higher than in <i>D. bardawil</i>, offering potential for industrial lycopene production.</p>

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Comparative characterization of CRTISO from D. salina and D. bardawil

  • 期刊论文

  • 发表日期：

    2025年9月20日

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  • Jia-Yuan Luo,
  • Qian-Xi Zheng,
  • Yu-Chen Xie,
  • Jv-Liang Dai,
  • Ibrahim Muazzam Mukhtar,
  • Ming-Hua Liang,
  • Hao-Hong Chen,
  • Jian-Guo Jiang

摘要

Dunaliella, known for its high salt tolerance and photosynthetic efficiency, plays a significant role in industrial and food production. This study presents a comparative analysis of the lycopene biosynthesis pathway in Dunaliella salina and Dunaliella bardawil. Genetic material from both species was extracted and expressed in a bacterial system, followed by HPLC, molecular docking, and phylogenetic analysis. The all-trans-lycopene content in the DsCRT strain was 1.26 ± 0.03 mg/g DCW, higher than that of the DbCRT strain. Molecular docking analysis showed that the binding affinity between DsCRTISO and the substrate was -11.18 kcal/mol, which was lower than the binding affinity of DbCRTISO (-10.08 kcal/mol), revealing that the catalytic activity of DsCRTISO was greater than that of DbCRTISO. Phylogenetic analysis confirmed a close relationship between D. salina and D. bardawil. HPLC showed that D. bardawil accumulate minimal β-carotene, likely due to the failure of specific gene regulatory mechanisms, despite sharing conserved CRTISO domains with D. salina. This study demonstrates that lycopene metabolism enzyme activity in D. salina is significantly higher than in D. bardawil, offering potential for industrial lycopene production.