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Abstract <p>This study examined the effects of mannan oligosaccharides (MOSs) on growth performance, hepatic and intestinal morphological structure, lipid accumulation, and lipid metabolism in hybrid groupers exposed to short-term (4 weeks) high-fat stress. Groupers averaging 101.59±0.85 g were allocated to three dietary groups: the C group (basal diet), the H group (high-fat diet), and the HM group (high-fat diet with 0.5% MOS). Compared with the C group, the H group presented significant decreases in the weight gain ratio (WGR), specific growth rate (SGR), and feed conversion ratio (FCR), along with increases in the hepatosomatic Index (HIS) and viscerosomatic index (VSI). MOS supplementation significantly reversed these effects. Morphological observations indicated that supplementation reduced lipid vacuolization in hepatocytes, decreased hepatic lipid accumulation, and alleviated intestinal damage. Transcriptome analysis revealed that in the intestine, key differentially expressed genes were enriched in lipid metabolism-related pathways. MOSs addition led to the downregulation of genes promoting lipid synthesis (<i>acaca</i>, <i>cd36</i>, <i>fas</i>, <i>scd-1</i>, and <i>hsd17b2</i> in the intestine and liver) and the upregulation of genes involved in lipolysis (<i>cpt-1</i>, <i>lpl</i>, <i>acacb</i>, <i>mgll</i>, <i>alox5a</i>, and <i>fxr</i> in the intestine and liver), thus reversing the adverse effects observed in the H group. These findings collectively indicate that supplementing the high-fat diet of hybrid groupers with 0.5% MOSs positively impacts their growth, liver and intestinal health, and lipid metabolism in these organs.</p>

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Effects of mannan-oligosaccharides on growth performance, hepatic and intestinal morphological structure, lipid accumulation, and lipid metabolism in hybrid groupers (Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀) under short-term high-fat stress

  • 期刊论文

  • 发表日期：

    2025年8月7日

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  • Zihan Chen,
  • Naiqi Liang,
  • Shifeng Wang,
  • Teng Chen,
  • Yiming Feng,
  • Yongcan Zhou,
  • Dongdong Zhang,
  • Ying Wu,
  • Yan Cai

摘要

Abstract

This study examined the effects of mannan oligosaccharides (MOSs) on growth performance, hepatic and intestinal morphological structure, lipid accumulation, and lipid metabolism in hybrid groupers exposed to short-term (4 weeks) high-fat stress. Groupers averaging 101.59±0.85 g were allocated to three dietary groups: the C group (basal diet), the H group (high-fat diet), and the HM group (high-fat diet with 0.5% MOS). Compared with the C group, the H group presented significant decreases in the weight gain ratio (WGR), specific growth rate (SGR), and feed conversion ratio (FCR), along with increases in the hepatosomatic Index (HIS) and viscerosomatic index (VSI). MOS supplementation significantly reversed these effects. Morphological observations indicated that supplementation reduced lipid vacuolization in hepatocytes, decreased hepatic lipid accumulation, and alleviated intestinal damage. Transcriptome analysis revealed that in the intestine, key differentially expressed genes were enriched in lipid metabolism-related pathways. MOSs addition led to the downregulation of genes promoting lipid synthesis (acaca, cd36, fas, scd-1, and hsd17b2 in the intestine and liver) and the upregulation of genes involved in lipolysis (cpt-1, lpl, acacb, mgll, alox5a, and fxr in the intestine and liver), thus reversing the adverse effects observed in the H group. These findings collectively indicate that supplementing the high-fat diet of hybrid groupers with 0.5% MOSs positively impacts their growth, liver and intestinal health, and lipid metabolism in these organs.