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<p>This experiment aimed to systematically evaluate the effects of <i>Clostridium butyricum</i> metabolite (CBM) on growth performance, antioxidant capacity, and intestinal health of bullfrogs induced by high dose of soybean meal. For the 8-week trial, three diets with identical crude protein (40%) and crude lipid (7%) contents were formulated. The control group was fed basic food, while experimental groups (CB1 and CB2) were supplemented with CBM at 0.1% and 0.2%, respectively. Results showed that survival was significantly higher and feed conversion was lower in the CB2 group (<i>P</i> &lt; 0.05); Elevated activities of lipase, α-amylase, and trypsin in CB1, and lipase activity was higher in CB2 (<i>P</i> &lt; 0.05); antioxidant indices, such as T-AOC, GSH, were elevated in both CB1 and CB2, while MDA levels decreased, with significant increases in CAT and SOD in CB1 (<i>P</i> &lt; 0.05); gene expression of <i>gsh-px</i>, <i>sod</i>, and <i>gstp</i> was upregulated in both CB1 and CB2, with increased <i>cat</i> expression in CB1 (<i>P</i> &lt; 0.05); inflammatory markers (<i>il-8</i>, <i>il-1β</i>, <i>tlr-5</i>, <i>tnf-α</i>) were reduced, and <i>il-10</i> expression increased in both groups, with enhanced <i>myd88</i> expression and decreased <i>nfκ-b</i> in CB2 (<i>P</i> &lt; 0.05); <i>Claudin-1</i>, <i>claudin-2</i> gene expression was upregulated in both groups (<i>P</i> &lt; 0.05); the intestinal microbiota structure improved, with significantly higher numbers of probiotics such as <i>Lactococcus</i>, <i>Cetobacterium</i>, and <i>Lachnospiraceae</i>. In summary, the addition of 0.2% of CBM to the diet of bullfrogs can significantly improve the survival rate of bullfrogs; when the addition of CBM is 0.1%, it can effectively alleviate the intestinal dysfunction of bullfrogs induced by the high soybean meal diet and further promote the overall health of bullfrogs.</p>

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Ameliorative effects of Clostridium butyricum metabolites on growth, oxidative stress, and intestinal health in bullfrogs fed high soybean meal diets

  • 期刊论文

  • 发表日期：

    2025年7月18日

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  • Xiongqi Wu,
  • Shuiquan Fu,
  • Yi Hu,
  • Bo Zhu,
  • Mengxi Yang,
  • Jihong Dai

摘要

This experiment aimed to systematically evaluate the effects of Clostridium butyricum metabolite (CBM) on growth performance, antioxidant capacity, and intestinal health of bullfrogs induced by high dose of soybean meal. For the 8-week trial, three diets with identical crude protein (40%) and crude lipid (7%) contents were formulated. The control group was fed basic food, while experimental groups (CB1 and CB2) were supplemented with CBM at 0.1% and 0.2%, respectively. Results showed that survival was significantly higher and feed conversion was lower in the CB2 group (P < 0.05); Elevated activities of lipase, α-amylase, and trypsin in CB1, and lipase activity was higher in CB2 (P < 0.05); antioxidant indices, such as T-AOC, GSH, were elevated in both CB1 and CB2, while MDA levels decreased, with significant increases in CAT and SOD in CB1 (P < 0.05); gene expression of gsh-px, sod, and gstp was upregulated in both CB1 and CB2, with increased cat expression in CB1 (P < 0.05); inflammatory markers (il-8, il-1β, tlr-5, tnf-α) were reduced, and il-10 expression increased in both groups, with enhanced myd88 expression and decreased nfκ-b in CB2 (P < 0.05); Claudin-1, claudin-2 gene expression was upregulated in both groups (P < 0.05); the intestinal microbiota structure improved, with significantly higher numbers of probiotics such as Lactococcus, Cetobacterium, and Lachnospiraceae. In summary, the addition of 0.2% of CBM to the diet of bullfrogs can significantly improve the survival rate of bullfrogs; when the addition of CBM is 0.1%, it can effectively alleviate the intestinal dysfunction of bullfrogs induced by the high soybean meal diet and further promote the overall health of bullfrogs.