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<p>Green-synthesized zinc nanoparticles (ZnNP) have attracted considerable attention for use as aqua-feed supplements. The effect of ZnNPs on cellular energy, amino acid metabolism, and gene expression of major histocompatibility-II (<i>MHC-II</i>) is poorly understood. Our study explored the effect of dietary ZnNPs supplementation on hepatic function and overall health status. Farmed Nile tilapia cultured in a hapa-culturing system were fed ZnNPs with two ascending doses of 30 and 60&#xa0;mg/kg dry feed for 8&#xa0;weeks. Liver function enzymes, hepatic energy, amino acid profiles, gene expression analysis, and hepatic morphometry were evaluated. Non-significant changes were observed in the estimated biochemical indices, and a significant decrease (<i>P</i> &lt; 0.05) in lactate dehydrogenase (LDH) <i>activity</i> was recorded in ZnNPs<sub>60</sub> mg/kg dry feed compared to the control non-supplemented fish. Hepatic energy adenosine triphosphate (ATP) and CoQ10 were increased (<i>P</i> &lt; 0.05) in the supplemented groups, whereas adenosine monophosphate (AMP) showed the opposite trend. The hepatic amino acid profile indicates the selectivity of dietary ZnNPs in modulating specific protein-metabolic pathways. A higher relative expression of <i>IL-1β</i>, along with no statistical changes in <i>IL-10</i>, <i>HSP70</i>, and <i>MHC-II</i>, was recorded. Normal liver histology and a significant dose-dependent increase in mean hepatocytic area were observed. Our results suggest that incorporating ZnNPs as a functional Nile tilapia supplement is beneficial. However, further comparative investigations of different fish species are required to determine the optimal dose.</p>

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Dietary algal zinc nanoparticles improve liver function and immunity in Nile tilapia, Oreochromis niloticus

  • 期刊论文

  • 发表日期：

    2025年7月30日

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  • Eman Zahran,
  • Fatma Ahmed,
  • Samia Elbahnaswy,
  • Omar A. Ahmed-Farid,
  • Ahmed I. A. Mansour,
  • Engy Risha,
  • Hanan H. Abdelhafeez,
  • Khalid M. Alkhodair,
  • Mahmoud G. El Sebaei

摘要

Green-synthesized zinc nanoparticles (ZnNP) have attracted considerable attention for use as aqua-feed supplements. The effect of ZnNPs on cellular energy, amino acid metabolism, and gene expression of major histocompatibility-II (MHC-II) is poorly understood. Our study explored the effect of dietary ZnNPs supplementation on hepatic function and overall health status. Farmed Nile tilapia cultured in a hapa-culturing system were fed ZnNPs with two ascending doses of 30 and 60 mg/kg dry feed for 8 weeks. Liver function enzymes, hepatic energy, amino acid profiles, gene expression analysis, and hepatic morphometry were evaluated. Non-significant changes were observed in the estimated biochemical indices, and a significant decrease (P < 0.05) in lactate dehydrogenase (LDH) activity was recorded in ZnNPs60 mg/kg dry feed compared to the control non-supplemented fish. Hepatic energy adenosine triphosphate (ATP) and CoQ10 were increased (P < 0.05) in the supplemented groups, whereas adenosine monophosphate (AMP) showed the opposite trend. The hepatic amino acid profile indicates the selectivity of dietary ZnNPs in modulating specific protein-metabolic pathways. A higher relative expression of IL-1β, along with no statistical changes in IL-10, HSP70, and MHC-II, was recorded. Normal liver histology and a significant dose-dependent increase in mean hepatocytic area were observed. Our results suggest that incorporating ZnNPs as a functional Nile tilapia supplement is beneficial. However, further comparative investigations of different fish species are required to determine the optimal dose.