Scholarlink

<p>Optimizing feeding strategies is crucial for enhancing the growth and survival of Pacific oyster (<i>Magallana gigas</i>) larvae. This study conducted a 15-day feeding experiment with three feeding regimens comprising <i>Isochrysis galbana</i> (<i>I. galbana</i>) alone, <i>Chlorella vulgaris</i> (<i>C. vulgaris</i>) alone, and a mixed feeding group. Results indicated that mixed feeding significantly improved survival rates and shell growth rates during the development from D-shaped larvae to pediveliger larvae (shell length 320&#xa0;µm) compared to single algal feeding. The shell length in the mixed-feeding group reached 247.94 ± 7.85&#xa0;µm (umbo larvae), showing a 1.2 and 1.3-fold increase compared to the <i>I. galbana</i> and <i>C. vulgaris</i> groups, respectively. Larvae from the mixed-feeding group exhibited significantly enhanced eye spot formation and settlement rates. Mixed feeding significantly upregulated <i>Mg</i>EAAT1 (1.6-fold) and <i>Mg</i>GDH (2.7-fold) expression compared to the <i>I. galbana</i> group. Spectrophotometric analysis revealed that mixed feeding elevated glutamate (Glu) levels by 1.03-fold (vs. C. vulgaris) and 1.1-fold (vs. I. galbana), while α-ketoglutarate (α-KG) increased 2.15-fold and 3.9-fold, respectively. This enhancement in amino acid transport and energy metabolism underscores the synergistic nutritional advantage of combining <i>I. galbana</i> and <i>C. vulgaris</i>, achieving superior larval performance unattainable with either species alone while reducing operational costs. These findings establish mixed algal feeding as an optimal strategy for commercial bivalve hatcheries, supporting both biological success and economic sustainability.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Mixed algal diets enhance glutamate metabolism to promote growth and development in Pacific oyster (Magallana gigas) larvae

  • 期刊论文

  • 发表日期：

    2025年7月21日

查看全文
  • Deliang Li,
  • Wenjing Ren,
  • Xueshu Zhang,
  • Yiran Si,
  • Chuanyan Yang,
  • Lingling Wang,
  • Linsheng Song

摘要

Optimizing feeding strategies is crucial for enhancing the growth and survival of Pacific oyster (Magallana gigas) larvae. This study conducted a 15-day feeding experiment with three feeding regimens comprising Isochrysis galbana (I. galbana) alone, Chlorella vulgaris (C. vulgaris) alone, and a mixed feeding group. Results indicated that mixed feeding significantly improved survival rates and shell growth rates during the development from D-shaped larvae to pediveliger larvae (shell length 320 µm) compared to single algal feeding. The shell length in the mixed-feeding group reached 247.94 ± 7.85 µm (umbo larvae), showing a 1.2 and 1.3-fold increase compared to the I. galbana and C. vulgaris groups, respectively. Larvae from the mixed-feeding group exhibited significantly enhanced eye spot formation and settlement rates. Mixed feeding significantly upregulated MgEAAT1 (1.6-fold) and MgGDH (2.7-fold) expression compared to the I. galbana group. Spectrophotometric analysis revealed that mixed feeding elevated glutamate (Glu) levels by 1.03-fold (vs. C. vulgaris) and 1.1-fold (vs. I. galbana), while α-ketoglutarate (α-KG) increased 2.15-fold and 3.9-fold, respectively. This enhancement in amino acid transport and energy metabolism underscores the synergistic nutritional advantage of combining I. galbana and C. vulgaris, achieving superior larval performance unattainable with either species alone while reducing operational costs. These findings establish mixed algal feeding as an optimal strategy for commercial bivalve hatcheries, supporting both biological success and economic sustainability.