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<p>Largemouth bass virus (LMBV), a Ranavirus pathogen, causes severe economic losses in aquaculture due to high mortality rates. To elucidate the immune regulatory mechanisms of largemouth bass against LMBV infection, this study combined histopathology, viral load quantification, transcriptome profiling, and qRT-PCR validation. Histological examination revealed that swollen liver cells with cytoplasmic vacuoles were observed, along with intestinal villus proliferation and expanded intestinal cavities at 48 and 72&#xa0;h post-infection. The viral load of kidney, liver, and spleen tissues of largemouth bass showed an increasing trend from 0 to 72&#xa0;h post-infection, with the highest and closest viral loads observed after 48 and 72&#xa0;h post-infection. Transcriptome analysis of LMBV-infected largemouth bass over a time course revealed that at 48&#xa0;h post-infection, most of the differentially expressed genes enriched in cytokine-cytokine receptor interaction pathways were highly upregulated. Comparative transcriptome analysis of 0 vs 48&#xa0;h post-infection (D-vs-H48 group) showed the top differentially expressed genes were mainly enriched in three key immune-related pathways: cytokine-cytokine receptor interaction, JAK-STAT signaling, and TNF signaling. Network analysis demonstrated interconnectivity between pathway components, suggesting coordinated regulation of the innate immune response. Finally, we validated the transcriptome results using qRT-PCR, confirming that expression of representative immunity-related genes increased significantly from baseline by 24&#xa0;h post-LMBV infection and beyond. Therefore, this study provides new insights into the innate immune responses of largemouth bass to LMBV and lays the foundation for future vaccine development and the breeding of new disease resistant varieties.</p>

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Transcriptomic profiling provides new insights into the intricate regulation of immune pathways in response to largemouth bass virus (LMBV) infection

  • 期刊论文

  • 发表日期：

    2025年7月4日

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  • Jing Chen,
  • Xue-Mei Yuan,
  • Lei Huang,
  • Xiao-Hong Huang,
  • Xian-Qi Peng,
  • Xia-Lian Bu,
  • Jin-Biao Jiao,
  • Jia-Yun Yao

摘要

Largemouth bass virus (LMBV), a Ranavirus pathogen, causes severe economic losses in aquaculture due to high mortality rates. To elucidate the immune regulatory mechanisms of largemouth bass against LMBV infection, this study combined histopathology, viral load quantification, transcriptome profiling, and qRT-PCR validation. Histological examination revealed that swollen liver cells with cytoplasmic vacuoles were observed, along with intestinal villus proliferation and expanded intestinal cavities at 48 and 72 h post-infection. The viral load of kidney, liver, and spleen tissues of largemouth bass showed an increasing trend from 0 to 72 h post-infection, with the highest and closest viral loads observed after 48 and 72 h post-infection. Transcriptome analysis of LMBV-infected largemouth bass over a time course revealed that at 48 h post-infection, most of the differentially expressed genes enriched in cytokine-cytokine receptor interaction pathways were highly upregulated. Comparative transcriptome analysis of 0 vs 48 h post-infection (D-vs-H48 group) showed the top differentially expressed genes were mainly enriched in three key immune-related pathways: cytokine-cytokine receptor interaction, JAK-STAT signaling, and TNF signaling. Network analysis demonstrated interconnectivity between pathway components, suggesting coordinated regulation of the innate immune response. Finally, we validated the transcriptome results using qRT-PCR, confirming that expression of representative immunity-related genes increased significantly from baseline by 24 h post-LMBV infection and beyond. Therefore, this study provides new insights into the innate immune responses of largemouth bass to LMBV and lays the foundation for future vaccine development and the breeding of new disease resistant varieties.