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<p>Hypoxia inducible factor (HIF-1α) is a key transcription factor in response to cellular hypoxia and plays an important role in various physiological processes. In this study, a hypoxia inducible factor (designated <i>Cg</i>HIF-1α) was identified from the Pacific oyster <i>Crassostrea gigas</i>, which contained one bHLH domain, two PAS domains, and one HIF-1 domain. After hypoxia stress treatment (DO = 1.3 ± 0.1&#xa0;mg/L), the mRNA expression of <i>Cg</i>HIF-1α in hepatopancreas was significantly increased at 6, 24, 48, and 72&#xa0;h compared with that in the control group (DO = 7.0 ± 0.1&#xa0;mg/L). The protein expression level of r<i>Cg</i>HIF-1α was also up-regulated under hypoxia stress, indicating that <i>Cg</i>HIF-1α could respond to hypoxia stress. The expression of anaerobic metabolism related genes including <i>Cg</i>HK, <i>Cg</i>PK, and <i>Cg</i>PEPCK in hepatopancreas were significantly increased at 48&#xa0;h under hypoxia stress. At 24&#xa0;h after hypoxia stress, the expression level of <i>Cg</i>PDK1 was significantly up-regulated, while that of <i>Cg</i>PDH was significantly down-regulated. After <i>Cg</i>HIF-1α was interfered with specific dsRNA, the expression levels of <i>Cg</i>HK, <i>Cg</i>PK, <i>Cg</i>PEPCK, and <i>Cg</i>PDK1 in hepatopancreas were decreased significantly; no significant change was detected in the expression level of <i>Cg</i>PDH. These showed that the expression of anaerobic metabolism related genes <i>Cg</i>HK, <i>Cg</i>PK, <i>Cg</i>PEPCK, and <i>Cg</i>PDK1 were directly regulated by <i>Cg</i>HIF-1α under hypoxia stress. The concentrations of pyruvate and acetyl-CoA remained unchanged after hypoxia stress, while the concentration of malate increased significantly after hypoxia stress for 72&#xa0;h. After interfering with the expression of <i>Cg</i>HIF-1α, the pyruvate concentration showed a marked decrease; the acetyl-CoA levels remained steadfastly consistent. There was an observable downward trend in the malate concentration, indicating that <i>Cg</i>HIF-1α participated in regulating the changes of anaerobic metabolic fluxes by regulating genes related to anaerobic metabolism under hypoxia stress. These results collectively suggested that <i>Cg</i>HIF-1α played a crucial role in regulating anaerobic metabolism in oysters under hypoxia stress.</p>

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Hypoxia inducible factor HIF-1α regulates anaerobic metabolism after hypoxia stress in Pacific oyster Crassostrea gigas

  • 期刊论文

  • 发表日期：

    2025年6月5日

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  • Jiabing Zhang,
  • Chang Liu,
  • Buxin Liu,
  • Lingling Wang,
  • Linsheng Song

摘要

Hypoxia inducible factor (HIF-1α) is a key transcription factor in response to cellular hypoxia and plays an important role in various physiological processes. In this study, a hypoxia inducible factor (designated CgHIF-1α) was identified from the Pacific oyster Crassostrea gigas, which contained one bHLH domain, two PAS domains, and one HIF-1 domain. After hypoxia stress treatment (DO = 1.3 ± 0.1 mg/L), the mRNA expression of CgHIF-1α in hepatopancreas was significantly increased at 6, 24, 48, and 72 h compared with that in the control group (DO = 7.0 ± 0.1 mg/L). The protein expression level of rCgHIF-1α was also up-regulated under hypoxia stress, indicating that CgHIF-1α could respond to hypoxia stress. The expression of anaerobic metabolism related genes including CgHK, CgPK, and CgPEPCK in hepatopancreas were significantly increased at 48 h under hypoxia stress. At 24 h after hypoxia stress, the expression level of CgPDK1 was significantly up-regulated, while that of CgPDH was significantly down-regulated. After CgHIF-1α was interfered with specific dsRNA, the expression levels of CgHK, CgPK, CgPEPCK, and CgPDK1 in hepatopancreas were decreased significantly; no significant change was detected in the expression level of CgPDH. These showed that the expression of anaerobic metabolism related genes CgHK, CgPK, CgPEPCK, and CgPDK1 were directly regulated by CgHIF-1α under hypoxia stress. The concentrations of pyruvate and acetyl-CoA remained unchanged after hypoxia stress, while the concentration of malate increased significantly after hypoxia stress for 72 h. After interfering with the expression of CgHIF-1α, the pyruvate concentration showed a marked decrease; the acetyl-CoA levels remained steadfastly consistent. There was an observable downward trend in the malate concentration, indicating that CgHIF-1α participated in regulating the changes of anaerobic metabolic fluxes by regulating genes related to anaerobic metabolism under hypoxia stress. These results collectively suggested that CgHIF-1α played a crucial role in regulating anaerobic metabolism in oysters under hypoxia stress.