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<p>Lipases serve as valuable feed additives in aquaculture by enhancing lipid digestion and nutrient absorption. Recognizing the need for more efficient and sustainable enzyme sources, cyanobacterial lipases for their unique catalytic properties and environmental compatibility were explored. In this study, a lipase gene from <i>Synechocystis</i> sp. PCC 6803 was successfully expressed in <i>Synechococcus elongatus</i> PCC 7942 under the control of the endogenous Pcpc promoter. The recombinant strain showed ~ twofold higher lipase activity (<i>p</i> &lt; 0.01) than wild-type, supported by increased protein expression on SDS-PAGE and enzymatic assays. When used as a feed additive for <i>Synechogobius hasta</i>, the transgenic algal supplement significantly improved growth performance, with a 21.7% increase in final body weight (<i>p</i> &lt; 0.01) and a significantly lower feed conversion ratio (FCR) (<i>p</i> &lt; 0.01) after 30&#xa0;days of feeding compared to control groups. These findings suggest that the cyanobacterial lipase may serve as a potential feed additive that can enhance both nutritional uptake and growth performance in aquaculture.</p>

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Heterologous expression of a Synechocystis sp. PCC 6803 lipase gene in Synechococcus elongatus PCC 7942

  • 期刊论文

  • 发表日期：

    2025年5月27日

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  • Xiaotong Ren,
  • Huaiyu Chen,
  • Mengting Wang,
  • Yongzhong Lu,
  • Fang Wang

摘要

Lipases serve as valuable feed additives in aquaculture by enhancing lipid digestion and nutrient absorption. Recognizing the need for more efficient and sustainable enzyme sources, cyanobacterial lipases for their unique catalytic properties and environmental compatibility were explored. In this study, a lipase gene from Synechocystis sp. PCC 6803 was successfully expressed in Synechococcus elongatus PCC 7942 under the control of the endogenous Pcpc promoter. The recombinant strain showed ~ twofold higher lipase activity (p < 0.01) than wild-type, supported by increased protein expression on SDS-PAGE and enzymatic assays. When used as a feed additive for Synechogobius hasta, the transgenic algal supplement significantly improved growth performance, with a 21.7% increase in final body weight (p < 0.01) and a significantly lower feed conversion ratio (FCR) (p < 0.01) after 30 days of feeding compared to control groups. These findings suggest that the cyanobacterial lipase may serve as a potential feed additive that can enhance both nutritional uptake and growth performance in aquaculture.