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<p><i>Crassostrea dianbaiensis</i>, a recently identified species with significant commercial potential, has been successfully hybridized with <i>Crassostrea iredalei</i>, demonstrating that fertilization can be bidirectional. Notably, only the DI hybrid lineage (<i>C. dianbaiensis</i>♀ × <i>C. iredalei</i>♂) achieves maturity. These DI hybrids display remarkable growth vigor and survival advantages over their parental strains. To further investigate the trait segregation patterns in the F<sub>2</sub> generation and the stable inheritance of heterosis, an intraspecific-crossing experiment was conducted with mature DI individuals, and place the juvenile shellfish in natural sea areas and artificial ponds for comparative aquaculture. The study involved crossing within <i>C. dianbaiensis</i>, <i>C. iredalei</i>, and mature DI individuals. The results indicated that F<sub>2</sub> hybrids could fertilize successfully and develop normally, but exhibited signs of outbreeding depression during the planktonic larval stage compared to the grandparental species. Genetic analysis using ITS1 markers classified F<sub>2</sub> individuals into three distinct categories: F<sub>2</sub>-I, F<sub>2</sub>-D, and F<sub>2</sub>-H. Water temperature fluctuations during the growth phase in Oushi’s ponds were more extreme than those observed in the Sanbaimen sea. On day 360, the ratio of F<sub>2</sub>-I, F<sub>2</sub>-D, and F<sub>2</sub>-H in Oushi’s ponds was approximately 1:1:4, contrasting with a 1:1:2 ratio in the Sanbaimen sea. This indicates that F<sub>2</sub>-H has a higher survival rate in Oushi’s ponds with drastic temperature changes. In addition, nearly all F<sub>2</sub>-D individuals showed phenotypic traits surpassing the F<sub>2</sub> average. Histological examination of gonads indicated that all three F<sub>2</sub> types were capable of gamete production, albeit with delayed gonadal development relative to age-matched grandparental species. In conclusion, both F<sub>2</sub>-H and F<sub>2</sub>-D exhibited heat tolerance and enhanced growth rates, respectively, suggesting their potential as new foundational breeding stocks for targeted development of strains adapted to large-scale aquaculture in southern China.</p>

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Trait segregation and growth characteristics of the F2 generation hybrids between Crassostrea dianbaiensis and C. iredalei

  • 期刊论文

  • 发表日期：

    2025年4月11日

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  • Jiangwei Li,
  • Ying Pan,
  • Qinggan Xing,
  • Jinhui Wu,
  • Haitao Ma,
  • Weitao Wan,
  • Chao Yue,
  • Zhongyu Wang,
  • Jun Li,
  • Ziniu Yu,
  • Yanping Qin,
  • Yuehuan Zhang

摘要

Crassostrea dianbaiensis, a recently identified species with significant commercial potential, has been successfully hybridized with Crassostrea iredalei, demonstrating that fertilization can be bidirectional. Notably, only the DI hybrid lineage (C. dianbaiensis♀ × C. iredalei♂) achieves maturity. These DI hybrids display remarkable growth vigor and survival advantages over their parental strains. To further investigate the trait segregation patterns in the F2 generation and the stable inheritance of heterosis, an intraspecific-crossing experiment was conducted with mature DI individuals, and place the juvenile shellfish in natural sea areas and artificial ponds for comparative aquaculture. The study involved crossing within C. dianbaiensis, C. iredalei, and mature DI individuals. The results indicated that F2 hybrids could fertilize successfully and develop normally, but exhibited signs of outbreeding depression during the planktonic larval stage compared to the grandparental species. Genetic analysis using ITS1 markers classified F2 individuals into three distinct categories: F2-I, F2-D, and F2-H. Water temperature fluctuations during the growth phase in Oushi’s ponds were more extreme than those observed in the Sanbaimen sea. On day 360, the ratio of F2-I, F2-D, and F2-H in Oushi’s ponds was approximately 1:1:4, contrasting with a 1:1:2 ratio in the Sanbaimen sea. This indicates that F2-H has a higher survival rate in Oushi’s ponds with drastic temperature changes. In addition, nearly all F2-D individuals showed phenotypic traits surpassing the F2 average. Histological examination of gonads indicated that all three F2 types were capable of gamete production, albeit with delayed gonadal development relative to age-matched grandparental species. In conclusion, both F2-H and F2-D exhibited heat tolerance and enhanced growth rates, respectively, suggesting their potential as new foundational breeding stocks for targeted development of strains adapted to large-scale aquaculture in southern China.