The development of alternative processing methods for Artemia biomass that maintains its nutritional properties and overcomes market restrictions and enables the creation of new diets to replace the use of live Artemia nauplii. Thus, this study consisted of two experiments: The first experiment evaluated six diets to feed Litopenaeus vannamei larvae in the Mysis stage. Diets consisted of CA \(_1\) , Artemia nauplii; APH, Artemia protein hydrolysate; APHC, Artemia protein hydrolysate concentrate; APHS, Artemia protein hydrolysate supernatant; DAB, dried artemia biomass; and LAB, lyophilized Artemia biomass. The results obtained in experiment 1 suggested that APH is a promising method for improving Artemia processing and that the correction of the feeding rate can improve the productive responses obtained with APH. To obtain greater reliability in the data obtained in experiment 1, a second experiment (Experiment 2) was performed using a control treatment = CA \(_2\) = Artemia nauplii., six APH levels in the diet of Litopenaeus vannamei, and two other treatments consisted of CC = microalgae Chaetoceros sp and CAC = Artemia + microalgae Chaetoceros sp. The results demonstrated that adjusting the number of daily feeds led to a decrease in total APH consumption without negatively impacting performance. The optimal feeding rate for Litopenaeus vannamei from Mysis I to III and PL I stages ranges between 118.5 and 178.5 \( \mu \) L \( ^{-1} \) day \( ^{-1} \) . We conclude that APH presents a viable alternative to live Artemia nauplii and serves as a potential feedstock for shrimp larvae farming. This is attributed to its increased total protein solubility and the percentage of protein solubility enabled by the processing, along with its dry matter:soluble protein ratio and the non-enzymatic inhibition of trypsin. The feeding frequency plays a decisive role in the utilization of this ingredient. Furthermore, a higher feeding frequency reduces the importance of dietary peptides in the overall profile.
