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<p>Tachykinin, a class of neuropeptides known for their direct interaction with vascular endothelial cells, plays a role in several essential physiological processes. This study aimed to investigate the role of tachykinin in the innate immunity of crustaceans, with a specific focus on hemocyte proliferation. RNA interference technology was employed to knock down tachykinin expression in <i>Scylla paramamosain</i> using tachykinin-dsRNA. The real-time quantitative PCR results demonstrated that the expression levels of <i>proPO</i>, <i>β-actin</i>, <i>astakine</i>, <i>crustin antimicrobial peptide (CAP)</i>, <i>JAK</i>, and <i>STAT</i> were downregulated following the knockdown of tachykinin, but <i>myosin</i>, <i>toll-like receptor (TLR)</i>, and <i>MCM7</i> expressions were upregulated. Following tachykinin knockdown, the enzyme activities of phenoloxidase (PO), peroxidase (POD), lysozyme (LZM), acid phosphatase (ACP), catalase (CAT), and total hemocyte counts were reduced in <i>S. paramamosain</i>. The flow cytometry data indicated that tachykinin knockdown enhanced the phagocytosis and apoptosis rate of crab hemocytes and decreased hemocyte proliferation. And survival analysis showed that tachykinin knockdown increased the mortality rate of <i>S. paramamosain</i> post-infection with <i>Vibrio alginolyticus</i>. These findings suggest that tachykinin may play a regulatory role in hemocyte proliferation and influence the innate immune response in <i>S. paramamosain</i>. Additionally, tachykinin significantly affected the survival rate of crabs even in the absence of <i>V. alginolyticus</i> challenge, suggesting its vital role for the survival of <i>S. paramamosain</i>. In conclusion, our study provides further insights into the important role of tachykinin in the innate immunity and hemocyte proliferation of <i>S</i>. <i>paramamosain</i>.</p>

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Tachykinin regulates the innate immunity and disease resistance in Scylla paramamosain

  • 期刊论文

  • 发表日期：

    2025年1月20日

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  • Yong Wang,
  • Fei Zhu

摘要

Tachykinin, a class of neuropeptides known for their direct interaction with vascular endothelial cells, plays a role in several essential physiological processes. This study aimed to investigate the role of tachykinin in the innate immunity of crustaceans, with a specific focus on hemocyte proliferation. RNA interference technology was employed to knock down tachykinin expression in Scylla paramamosain using tachykinin-dsRNA. The real-time quantitative PCR results demonstrated that the expression levels of proPO, β-actin, astakine, crustin antimicrobial peptide (CAP), JAK, and STAT were downregulated following the knockdown of tachykinin, but myosin, toll-like receptor (TLR), and MCM7 expressions were upregulated. Following tachykinin knockdown, the enzyme activities of phenoloxidase (PO), peroxidase (POD), lysozyme (LZM), acid phosphatase (ACP), catalase (CAT), and total hemocyte counts were reduced in S. paramamosain. The flow cytometry data indicated that tachykinin knockdown enhanced the phagocytosis and apoptosis rate of crab hemocytes and decreased hemocyte proliferation. And survival analysis showed that tachykinin knockdown increased the mortality rate of S. paramamosain post-infection with Vibrio alginolyticus. These findings suggest that tachykinin may play a regulatory role in hemocyte proliferation and influence the innate immune response in S. paramamosain. Additionally, tachykinin significantly affected the survival rate of crabs even in the absence of V. alginolyticus challenge, suggesting its vital role for the survival of S. paramamosain. In conclusion, our study provides further insights into the important role of tachykinin in the innate immunity and hemocyte proliferation of S. paramamosain.