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<p><i>Vibrio alginolyticus</i> is associated with severe infections in marine fish and immunocompromised people. This study investigated the prevalence, genetic typing, associated virulence traits, unveiling resistance genes, and the pathogenicity of <i>V. alginolyticus</i> isolated from sea bream. A total of 200 sea bream (<i>Sparus aurata</i>) were gathered from secluded farms in Port Said, Egypt. Subsequently, clinical, postmortem, and bacteriological analyses, including morphological, culture, biochemical characterization, and PCR-based collagenase gene detection, were performed. The isolated strains were tested for <i>collagenase</i> gene sequencing, antibiogram, pathogenicity, and PCR monitoring of virulence and resistance genes. Herein, <i>V. alginolyticus</i> was found in 14% (28/200) of the collected sea bream, exclusively from the moribund fish. Moreover, the collagenase phylogeny revealed that the tested<i> V. alginolyticus</i> isolates have a distinguished genetic homogeneity with other <i>V. alginolyticus</i> isolates from China, Japan, and the USA. Moreover, the most predominant virulence genes in the tested <i>V. alginolyticus</i> were the <i>collagenase</i> (100%), <i>trh</i> (90.4%), <i>tlh</i> (59.6%), and <i>tdh</i> (21.1%) genes. In this study, 28.8% of the obtained <i>V. alginolyticus</i> strains were multidrug-resistant (MDR) to five classes and encoded <i>bla</i><sub>TEM</sub>,<i> aad</i>A, <i>tet</i>A, <i>bla</i><sub>OXA</sub>, and<i> sul</i>1 genes. Likewise, 15.4% of <i>V</i>. <i>alginolyticus</i> were MDR to four classes and encoded <i>sul</i>1 and <i>tet</i>B genes. As well, 7.7% were MDR to six classes and encoded <i>bla</i><sub>TEM</sub>,<i> tet</i>M, <i>sul</i>1, and <i>bla</i><sub>OXA</sub> genes. Using the cumulative mortality curve, a high mortality rate (87%) was recorded within 7&#xa0;days in the challenged <i>Tilapia zillii</i> with a virulent <i>V. alginolyticus</i> strain. Concisely, this investigation draws attention to the evolution of MDR <i>V. alginolyticus</i> in sea bream, instructing a health problem. The <i>collagenase</i>, <i>trh</i>, and <i>tlh</i> virulence-related genes and the <i>sul</i>1,<i> bla</i><sub>TEM</sub>,<i> aad</i>A,<i> tet</i>A,<i>&#xa0;bla</i><sub>OXA</sub>, and<i>&#xa0;tet</i>B or <i>tet</i>M resistance genes commonly accompanied the evolving MDR <i>V. alginolyticus</i> in sea bream.</p>

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The evolving multidrug-resistant V. alginolyticus in sea bream commonly harbored collagenase, trh, and tlh virulence genes and sul1, blaTEM, aadA, tetA, blaOXA, and tetB or tetM resistance genes

  • 期刊论文

  • 发表日期：

    2025年1月10日

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  • Abdelazeem M. Algammal,
  • Mahmoud Mabrok,
  • Khyreyah J. Alfifi,
  • Saad Alghamdi,
  • Dalia M. Alammari,
  • Madeha O. I. Ghobashy,
  • Mohammad Y. Alshahrani,
  • Azza S. El-Demerdash,
  • El-Sayed Hemdan Eissa,
  • Aya M. Elalamy,
  • Reham M. El-Tarabili

摘要

Vibrio alginolyticus is associated with severe infections in marine fish and immunocompromised people. This study investigated the prevalence, genetic typing, associated virulence traits, unveiling resistance genes, and the pathogenicity of V. alginolyticus isolated from sea bream. A total of 200 sea bream (Sparus aurata) were gathered from secluded farms in Port Said, Egypt. Subsequently, clinical, postmortem, and bacteriological analyses, including morphological, culture, biochemical characterization, and PCR-based collagenase gene detection, were performed. The isolated strains were tested for collagenase gene sequencing, antibiogram, pathogenicity, and PCR monitoring of virulence and resistance genes. Herein, V. alginolyticus was found in 14% (28/200) of the collected sea bream, exclusively from the moribund fish. Moreover, the collagenase phylogeny revealed that the tested V. alginolyticus isolates have a distinguished genetic homogeneity with other V. alginolyticus isolates from China, Japan, and the USA. Moreover, the most predominant virulence genes in the tested V. alginolyticus were the collagenase (100%), trh (90.4%), tlh (59.6%), and tdh (21.1%) genes. In this study, 28.8% of the obtained V. alginolyticus strains were multidrug-resistant (MDR) to five classes and encoded blaTEM, aadA, tetA, blaOXA, and sul1 genes. Likewise, 15.4% of V. alginolyticus were MDR to four classes and encoded sul1 and tetB genes. As well, 7.7% were MDR to six classes and encoded blaTEM, tetM, sul1, and blaOXA genes. Using the cumulative mortality curve, a high mortality rate (87%) was recorded within 7 days in the challenged Tilapia zillii with a virulent V. alginolyticus strain. Concisely, this investigation draws attention to the evolution of MDR V. alginolyticus in sea bream, instructing a health problem. The collagenase, trh, and tlh virulence-related genes and the sul1, blaTEM, aadA, tetA, blaOXA, and tetB or tetM resistance genes commonly accompanied the evolving MDR V. alginolyticus in sea bream.