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<p>In order to investigate the effects of dietary <i>Porphyra yezoensis</i> polysaccharide (PP) on the non-specific immunity of spotted sea bass (<i>Lateolabrax maculatus</i>), 360 spotted sea bass (10.53 ± 0.02&#xa0;g) were randomly divided into six groups and fed six different doses of PP (0, 3, 6, 9, 12, and 15&#xa0;g/kg), respectively. The experiment lasted for 52&#xa0;days. The results of non-specific immune indexes showed that compared with the control group, the activities of acid phosphatase (ACP) and alkaline phosphatase (AKP) in serum of the 6 and 9&#xa0;g/kg groups were significantly increased, and the activity of lysozyme (LZM) in each experimental groups was also significantly increased; in the liver, ACP activity in 3 and 6&#xa0;g/kg groups was significantly increased, and LZM activity in 9 and 12&#xa0;g/kg groups was significantly increased; in the head kidney, ACP and LZM activities in 9&#xa0;g/kg group were significantly increased; in the spleen, ACP, AKP, and LZM activities were significantly increased in the 9&#xa0;g/kg group. The transcriptomic sequencing results of the head kidney showed that PP could improve the immunity of spotted sea bass by regulating the expression of MHC Class I antigen (MHC1), glucocorticoid receptor (NR3C1), small subunit ribosomal protein S3Ae (RPS3A), heme oxygenase 1 (HMOX1), and ferritin heavy chain (FTH1) genes. The results of this study indicate that 9–12&#xa0;g/kg PP can improve the non-specific immunity of spotted sea bass and provide a theoretical basis for the application of PP in aquatic animal feed.</p>

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Transcriptome analysis of the effects of Porphyra yezoensis polysaccharide on non-specific immunity of spotted sea bass, Lateolabrax maculatus

  • 期刊论文

  • 发表日期：

    2025年1月2日

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  • Hao Lin,
  • Sishun Zhou,
  • Jianrong Ma,
  • Lumin Kong,
  • Zhangfan Huang,
  • Longhui Liu,
  • Yanbo Zhao,
  • Yi Lin,
  • Zhongbao Li

摘要

In order to investigate the effects of dietary Porphyra yezoensis polysaccharide (PP) on the non-specific immunity of spotted sea bass (Lateolabrax maculatus), 360 spotted sea bass (10.53 ± 0.02 g) were randomly divided into six groups and fed six different doses of PP (0, 3, 6, 9, 12, and 15 g/kg), respectively. The experiment lasted for 52 days. The results of non-specific immune indexes showed that compared with the control group, the activities of acid phosphatase (ACP) and alkaline phosphatase (AKP) in serum of the 6 and 9 g/kg groups were significantly increased, and the activity of lysozyme (LZM) in each experimental groups was also significantly increased; in the liver, ACP activity in 3 and 6 g/kg groups was significantly increased, and LZM activity in 9 and 12 g/kg groups was significantly increased; in the head kidney, ACP and LZM activities in 9 g/kg group were significantly increased; in the spleen, ACP, AKP, and LZM activities were significantly increased in the 9 g/kg group. The transcriptomic sequencing results of the head kidney showed that PP could improve the immunity of spotted sea bass by regulating the expression of MHC Class I antigen (MHC1), glucocorticoid receptor (NR3C1), small subunit ribosomal protein S3Ae (RPS3A), heme oxygenase 1 (HMOX1), and ferritin heavy chain (FTH1) genes. The results of this study indicate that 9–12 g/kg PP can improve the non-specific immunity of spotted sea bass and provide a theoretical basis for the application of PP in aquatic animal feed.