<p>Choline, a constituent of lecithin, is a water-soluble nutrient that plays a crucial role in the transport and utilization of hepatic fat. In aquaculture, choline is frequently regarded as a pivotal nutrient for the maintenance of fish’s regular growth and metabolic processes. Fish subjected to a choline-deficient or inadequate diet exhibit a range of symptoms, including stunted growth, diminished feed efficiency, and compromised survival rates or lipid metabolism. The beneficial effects of dietary choline, including promotion of growth, lipid metabolism, feed utilization, flesh quality, and antioxidant capacity, as well as regulation of immunity and antibacterial ability in fish have been well demonstrated in various fish species. Nevertheless, various issues including the administration pathways, as well as the interaction between choline and intestinal microbial colonization should be considered. In this review, we compile the present state of understanding regarding the physicochemical characteristics and physiological functions of choline, gather the most recent research discoveries concerning the impact of choline, and deliberate on the deficiencies in the utilization of choline in fish cultivation, with the goal of guaranteeing the production of robust fish with exceptional quality standards and optimized welfare conditions.</p>