Dietary Macleaya cordata extract supplementation reduced high-fat–induced damage of antioxidant capacity and immune function, and alleviated the disorder of glycolipid metabolism in Marsupenaeus japonicus

Our study aims to investigate whether Macleaya cordata extracts (MCE) could alleviate the adverse reactions caused by a high-fat (HF) diet in Marsupenaeus japonicus. Three experimental treatments including a control group (CG, 8.06% lipid), HF group (14.76% lipid), and HF group supplemented with 1200 mg/kg Macleaya cordata extract (HFG + MCE) were designed. The results showed that growth performance was not influenced (P > 0.05) by different diet treatments after a 42-day feeding trial. Compared with the control group, the HF group significantly decreased (P < 0.05) the activities of antioxidant enzymes in the serum and hepatopancreas. Expression of antioxidant-related genes was synchronously decreased (P < 0.05), while hsp70 gene expression was increased (P < 0.05) in the hepatopancreas. Hepatopancreatic glycolipid metabolism was upregulated (P < 0.05), which could be identified by lipid/glycogen content and gene expression. Compared with the HC group, the HC + MCE group significantly alleviated HC-induced oxidative stress, which was identified by the increased (P < 0.05) antioxidant enzyme activities and gene expression. Meanwhile, hepatopancreatic glycolipid metabolism was downregulated (P < 0.05). In conclusion, dietary supplementation with MCE could alleviate HF-induced damage of antioxidant capacity and immune function and alleviate the disorder of glycolipid metabolism in M. japonicus.