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<p><i>Dermacentor marginatus</i> is an important disease vector in China, yet current control methods remain heavily reliant on chemical acaricides. This study evaluated the vaccine potential of Ferritin 2 from <i>D. marginatus</i> (<i>Dm</i>Fer2). The <i>DmFer</i>2 gene was cloned, expressed as a recombinant <i>Dm</i>Fer2 (r<i>Dm</i>Fer2) in a prokaryotic expression system, and subsequently used to immunize rabbits. Immunization elicited high anti-r<i>Dm</i>Fer2 antibody titers, which peaked at a detectable maximum of 1:25,600 on day 35, and significantly reduced tick fecundity and altered tick feeding kinetics in challenge trials. Specifically, r<i>Dm</i>Fer2 vaccination resulted in prolonged engorgement cycle (8.25% relative extension), and reductions in the oviposition rate (19.64%), egg mass weight (43.74%), and hatching rate (38.5%), leading to an overall anti-tick efficacy of 82.1%. These results demonstrate, for the first time, that r<i>Dm</i>Fer2 is a highly effective vaccine candidate against <i>D. marginatus</i>. Its protective effects are primarily attributed to disrupting tick iron metabolism and interfering with tick feeding dynamics, and provide a theoretical and experimental foundation for the development of sustainable tick control strategies.</p>

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High protective efficacy of a recombinant Fer2 vaccine against Dermacentor marginatus infestations

  • 期刊论文

  • 发表日期：

    2026年5月6日

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  • Ying Ma,
  • Jiancheng Jiang,
  • Yanying Min,
  • Mingjie Hou,
  • Wenwen He,
  • Licui Wen,
  • Wenyu Shi,
  • Ercha Hu,
  • Qingyong Guo

摘要

Dermacentor marginatus is an important disease vector in China, yet current control methods remain heavily reliant on chemical acaricides. This study evaluated the vaccine potential of Ferritin 2 from D. marginatus (DmFer2). The DmFer2 gene was cloned, expressed as a recombinant DmFer2 (rDmFer2) in a prokaryotic expression system, and subsequently used to immunize rabbits. Immunization elicited high anti-rDmFer2 antibody titers, which peaked at a detectable maximum of 1:25,600 on day 35, and significantly reduced tick fecundity and altered tick feeding kinetics in challenge trials. Specifically, rDmFer2 vaccination resulted in prolonged engorgement cycle (8.25% relative extension), and reductions in the oviposition rate (19.64%), egg mass weight (43.74%), and hatching rate (38.5%), leading to an overall anti-tick efficacy of 82.1%. These results demonstrate, for the first time, that rDmFer2 is a highly effective vaccine candidate against D. marginatus. Its protective effects are primarily attributed to disrupting tick iron metabolism and interfering with tick feeding dynamics, and provide a theoretical and experimental foundation for the development of sustainable tick control strategies.