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<p>This study investigates the strain F-11, a ureolytic <i>Vibrio</i> isolated from seawater, and its potential for microbially induced calcium carbonate precipitation (MICP). Based on morphological and biochemical characteristics together with 16S rRNA gene sequence analysis, strain F-11 showed the highest similarity to <i>Vibrio alginolyticus</i> (96.79%) and was therefore conservatively designated as <i>Vibrio</i> sp. F-11. Urease activity measured by Nessler-based ammonium assay was examined at different temperatures (24, 30, 36&#xa0;°C) and pH values (6, 8, 10). Maximum ureolytic activity was observed between days 3 and 5at 30&#xa0;°C and pH 8, reaching approximately 0.58 and 0.57&#xa0;µmol&#xa0;min⁻<sup>1</sup>&#xa0;mg⁻<sup>1</sup> protein, respectively, whereas suboptimal conditions significantly reduced activity. Calcium carbonate precipitation occurred only on urea-CaCl<sub>2</sub> agar at pH 8; here, opaque white mineral deposits accumulated around bacterial colonies. Microscopic observations revealed rosette-like and spherical CaCO<sub>3</sub> crystals, while SEM images revealed compact, spherical precipitates. EDS spectra dominated by Ca, C, and O peaks confirmed the formation of CaCO<sub>3</sub> phases. In general, <i>Vibrio</i> sp. strain F-11 demonstrated effective biomineralization capacity and represents a promising MICP agent for future marine and cementitious biotechnological applications.</p>

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Seawater-derived ureolytic Vibrio sp. strain F-11 as a microbial agent for calcium carbonate biomineralization

  • 期刊论文

  • 发表日期：

    2026年5月8日

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  • Ecren Uzun Yaylacı,
  • Ayşe Kara,
  • Demet Ülkü Gülpınar Sekban,
  • Murat Yaylacı

摘要

This study investigates the strain F-11, a ureolytic Vibrio isolated from seawater, and its potential for microbially induced calcium carbonate precipitation (MICP). Based on morphological and biochemical characteristics together with 16S rRNA gene sequence analysis, strain F-11 showed the highest similarity to Vibrio alginolyticus (96.79%) and was therefore conservatively designated as Vibrio sp. F-11. Urease activity measured by Nessler-based ammonium assay was examined at different temperatures (24, 30, 36 °C) and pH values (6, 8, 10). Maximum ureolytic activity was observed between days 3 and 5at 30 °C and pH 8, reaching approximately 0.58 and 0.57 µmol min⁻1 mg⁻1 protein, respectively, whereas suboptimal conditions significantly reduced activity. Calcium carbonate precipitation occurred only on urea-CaCl2 agar at pH 8; here, opaque white mineral deposits accumulated around bacterial colonies. Microscopic observations revealed rosette-like and spherical CaCO3 crystals, while SEM images revealed compact, spherical precipitates. EDS spectra dominated by Ca, C, and O peaks confirmed the formation of CaCO3 phases. In general, Vibrio sp. strain F-11 demonstrated effective biomineralization capacity and represents a promising MICP agent for future marine and cementitious biotechnological applications.