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<p>Two novel anaerobic bacterial strains, designated JC010<sup>T</sup> and JC022<sup>T</sup>, were isolated from seawater samples collected in the Yongle Blue Hole, South China Sea. Phylogenetic analysis based on 16S rRNA gene sequences placed both strains within a distinct clade of the genus <i>Maridesulfovibrio</i>. Comparative 16S rRNA gene sequencing showed that the sequence similarity between the two isolates was 98.42%, while their similarities to all validly published <i>Maridesulfovibrio</i> species were below the 98.65% species threshold (JC010<sup>T</sup>: &lt; 97.9%; JC022<sup>T</sup>: &lt; 97.36%). Cells of both strains were Gram-stain-negative, motile vibrios with polar flagella, and exhibited obligately anaerobic metabolism. Strain-specific growth parameters were characterized: JC010<sup>T</sup> proliferated at 4–37&#xa0;°C (optimum 28&#xa0;°C), pH 4.0–9.0 (optimum 7.0) and in the presence of 1.0–5.5% NaCl (optimum 4.0%), whereas JC022<sup>T</sup> demonstrated a narrower temperature range (10–37&#xa0;°C; optimum 28 ℃), similar pH tolerance (4.0–8.0; optimum 7.0), and a distinct salinity preference (2.5–6.0% NaCl; optimum 3.0%). Chemotaxonomic analyses revealed that while the two strains exhibited several common characteristics, including menaquinone-7 (MK-7) as the predominant respiratory quinone, and major fatty acids comprising summed features 3 (C16:1 ω6c/ω7c), 9 (iso-C17:1 ω9c/C16:0 10-methyl), C16:0, and iso-C15:0, certain differences between them were also identified. The conserved polar lipid profiles included one phosphatidylcholine (PC), one phosphatidylethanolamine (PE), one phosphatidylglycerol (PG), and two phospholipids (PL1-2). Notably, JC022<sup>T</sup> contained three additional unidentified lipids that were not present in JC010<sup>T</sup>. Genomic comparisons further reinforced their taxonomic distinctness. The DNA G+C content was 49.8&#xa0;mol% for JC010<sup>T</sup> and 48.0&#xa0;mol% for JC022<sup>T</sup>. Based on the polyphasic characterization, we propose two novel species: <i>Maridesulfovibrio caeruleilacunae</i> sp. nov. (type strain JC010<sup>T</sup> = JCM 39061<sup>T</sup> = MCCC 1K03847<sup>T</sup>) and <i>Maridesulfovibrio oucae</i> sp. nov. (type strain JC022<sup>T</sup> = JCM 39062<sup>T</sup> = MCCC 1K03848<sup>T</sup>).</p>

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Maridesulfovibrio caeruleilacunae sp. nov. and Maridesulfovibrio oucae sp. nov., two sulfate-reducing bacteria isolated from Yongle Blue Hole of the South China Sea

  • 期刊论文

  • 发表日期：

    2026年4月30日

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  • Wei Dai,
  • Yifan Zhuang,
  • Wen Sun,
  • Tianyu Fu,
  • Chao Jia,
  • Liang Fu,
  • Peng Yao,
  • Zuosheng Yang,
  • Shun Zhou,
  • Xiaochong Shi,
  • Xiao-Hua Zhang

摘要

Two novel anaerobic bacterial strains, designated JC010T and JC022T, were isolated from seawater samples collected in the Yongle Blue Hole, South China Sea. Phylogenetic analysis based on 16S rRNA gene sequences placed both strains within a distinct clade of the genus Maridesulfovibrio. Comparative 16S rRNA gene sequencing showed that the sequence similarity between the two isolates was 98.42%, while their similarities to all validly published Maridesulfovibrio species were below the 98.65% species threshold (JC010T: < 97.9%; JC022T: < 97.36%). Cells of both strains were Gram-stain-negative, motile vibrios with polar flagella, and exhibited obligately anaerobic metabolism. Strain-specific growth parameters were characterized: JC010T proliferated at 4–37 °C (optimum 28 °C), pH 4.0–9.0 (optimum 7.0) and in the presence of 1.0–5.5% NaCl (optimum 4.0%), whereas JC022T demonstrated a narrower temperature range (10–37 °C; optimum 28 ℃), similar pH tolerance (4.0–8.0; optimum 7.0), and a distinct salinity preference (2.5–6.0% NaCl; optimum 3.0%). Chemotaxonomic analyses revealed that while the two strains exhibited several common characteristics, including menaquinone-7 (MK-7) as the predominant respiratory quinone, and major fatty acids comprising summed features 3 (C16:1 ω6c/ω7c), 9 (iso-C17:1 ω9c/C16:0 10-methyl), C16:0, and iso-C15:0, certain differences between them were also identified. The conserved polar lipid profiles included one phosphatidylcholine (PC), one phosphatidylethanolamine (PE), one phosphatidylglycerol (PG), and two phospholipids (PL1-2). Notably, JC022T contained three additional unidentified lipids that were not present in JC010T. Genomic comparisons further reinforced their taxonomic distinctness. The DNA G+C content was 49.8 mol% for JC010T and 48.0 mol% for JC022T. Based on the polyphasic characterization, we propose two novel species: Maridesulfovibrio caeruleilacunae sp. nov. (type strain JC010T = JCM 39061T = MCCC 1K03847T) and Maridesulfovibrio oucae sp. nov. (type strain JC022T = JCM 39062T = MCCC 1K03848T).